Danielle Schmitt*
/in /by SOFIA
Email Address:
dlschmitt@chem.ucla.edu
Office Phone Number:
(310) 825-3767
Lab website
Lab website
Office Address:
5040A Young Hall
Assistant Professor, Chemistry & Biochemistry
The Schmitt Lab is focused on answering the question: How does the cell organize essential processes like metabolism and signaling to coordinate cell function and fate? To answer this question, the Schmitt Lab takes a multi-disciplinary approach to identify mechanisms for sub-cellular compartmentation of metabolic processes, including signaling networks regulating metabolism and metabolites. We develop genetically encoded fluorescent protein-based biosensors to study cellular activity in single cells in real time with high spatial and temporal resolution using live cell fluorescence microscopy. We use these biosensors to (1) study kinase signaling networks which regulate metabolic processes and (2) interrogate sub-cellular dynamics of metabolites. We are interested in understanding how metabolism is spatiotemporally regulated in the cell, and how diseases of metabolic origin, like cancer and inborn errors of metabolism perturb this regulation.
A selected list of publications:
Schmitt, D.L., Curtis, S.D., Lyons, A.C., Chen, M., He, C.Y., Mehta, S., Shaw, R.J., and Zhang, J. Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter. Nature Communications, 13 (2022)
Schmitt, D.L., Dranchak, P., Parajuli, P., Blivis, D., Voss, T., Kohnhorst, C.L., Kyoung, M., Inglese, J., and An, S., High-content quantitative high-throughput screening identifies a cell cycle-associated signaling cascade that regulates a multienzyme metabolic assembly for glucose metabolism. BioRxiv, 2022
Jeon, M. Schmitt, D.L., Kyoung, M., and An, S. Functional oscillation of a multienzyme glucosome assembly during cell cycle progression. BioRxiv, 2022
Schmitt, D.L., Mehta, S., Zhang, J. Illuminating the kinome: visualizing real-time kinase activity in biological systems using genetically encoded fluorescent protein-based biosensors. Current Opinion in Chemical Biology, 54, 63-69, 2020
Ohadi, D., Schmitt, D.L., Calabrese, B., Halpain, S., Zhang, J., and Rangamani, P. Computational modeling reveals frequency modulation of calcium-cAMP/PKA pathway in dendritic spines, Biophysical Journal, 117, 1963-1980, 2019
Schmitt, D.L., Sundaram, A., Jeon, M., Luu, B.T., and An, S. Spatial regulation of de novo purine biosynthesis by Akt-independent PDK1 signaling pathways, PLOS ONE, 13, e0195989, 2018
Schmitt, D.L., An, S. Spatial Organization of Metabolic Enzyme Complexes in Cells, Biochemistry, 56, 3184 3196, 2017
Kohnhorst, C. L., Kyoung, M., Jeon, M., Schmitt, D. L., Kennedy, E. L., Ramirez, J., Bracey, S. M., Luu, B.T., Russell, S. J., and An, S. Identification of a Multienzyme Complex for Glucose Metabolism in Living Cells, Journal of Biological Chemistry, 292, 9191-9203, 2017
Schmitt, D. L., Cheng, Y., Park, J., and An, S. Sequestration-mediated downregulation of de novo purine biosynthesis by AMPK, ACS Chemical Biology, 11, 1917-24, 2016
Kohnhorst, C. L., Schmitt, D.L., Sundaram, A., and An, S. Subcellular functions of proteins under fluorescence single-cell microscopy, BBA Proteins and Proteomics, 1864, 77-84, 2015
Klee, T., Masterson, T., Miller, B., Barrasso, E., Ball, J., Lepkowiz, R., West, J., Haley, J. E., Schmitt, D. L., Flikkema, J. L., Cooper, T. M., Ruiz-Morales, Y., and Mullens, O. C. Triplet Electronic Spin States of Crude Oils and Asphaltenes, Energy and Fuels, 25, 2065-75, 2011