Danielle Schmitt


Danielle Schmitt, Ph.D.

Office Address:
5040A Young Hall

Affiliations

Assistant Professor, Chemistry & Biochemistry

Research Interests

The Schmitt Lab is focused on answering the question: How does the cell organize essential processes like metabolism and signaling to coordinate cell function and fate? To answer this question, the Schmitt Lab takes a multi-disciplinary approach to identify mechanisms for sub-cellular compartmentation of metabolic processes, including signaling networks regulating metabolism and metabolites. We develop genetically encoded fluorescent protein-based biosensors to study cellular activity in single cells in real time with high spatial and temporal resolution using live cell fluorescence microscopy. We use these biosensors to (1) study kinase signaling networks which regulate metabolic processes and (2) interrogate sub-cellular dynamics of metabolites. We are interested in understanding how metabolism is spatiotemporally regulated in the cell, and how diseases of metabolic origin, like cancer and inborn errors of metabolism perturb this regulation.

Publications

A selected list of publications:

 

Schmitt, D.L., Curtis, S.D., Lyons, A.C., Chen, M., He, C.Y., Mehta, S., Shaw, R.J., and Zhang, J. Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter. Nature Communications, 13 (2022)

Schmitt, D.L., Dranchak, P., Parajuli, P., Blivis, D., Voss, T., Kohnhorst, C.L., Kyoung, M., Inglese, J., and An, S., High-content quantitative high-throughput screening identifies a cell cycle-associated signaling cascade that regulates a multienzyme metabolic assembly for glucose metabolismBioRxiv, 2022

Jeon, M. Schmitt, D.L., Kyoung, M., and An, S. Functional oscillation of a multienzyme glucosome assembly during cell cycle progressionBioRxiv, 2022

Ohadi, D., Schmitt, D.L., Calabrese, B., Halpain, S., Zhang, J., and Rangamani, P. Computational modeling reveals frequency modulation of calcium-cAMP/PKA pathway in dendritic spinesBiophysical Journal, 117, 1963-1980, 2019

Schmitt, D.L., Sundaram, A., Jeon, M., Luu, B.T., and An, S. Spatial regulation of de novo purine biosynthesis by Akt-independent PDK1 signaling pathwaysPLOS ONE, 13, e0195989, 2018

Schmitt, D.L., An, S. Spatial Organization of Metabolic Enzyme Complexes in CellsBiochemistry, 56, 3184 3196, 2017

Kohnhorst, C. L., Kyoung, M., Jeon, M., Schmitt, D. L., Kennedy, E. L., Ramirez, J., Bracey, S. M., Luu, B.T., Russell, S. J., and An, S. Identification of a Multienzyme Complex for Glucose Metabolism in Living CellsJournal of Biological Chemistry, 292, 9191-9203, 2017

Schmitt, D. L., Cheng, Y., Park, J., and An, S. Sequestration-mediated downregulation of de novo purine biosynthesis by AMPKACS Chemical Biology, 11, 1917-24, 2016

Kohnhorst, C. L., Schmitt, D.L., Sundaram, A., and An, S. Subcellular functions of proteins under fluorescence single-cell microscopyBBA Proteins and Proteomics, 1864, 77-84, 2015

Klee, T., Masterson, T., Miller, B., Barrasso, E., Ball, J., Lepkowiz, R., West, J., Haley, J. E., Schmitt, D. L., Flikkema, J. L., Cooper, T. M., Ruiz-Morales, Y., and Mullens, O. C. Triplet Electronic Spin States of Crude Oils and AsphaltenesEnergy and Fuels, 25, 2065-75, 2011