Guillaume F. Chanfreau*
Publications:
Chanfreau GF. The Goldilocks state of transcriptional readthrough for chimeric splicing. Transcription. 2026 Apr 10; 1-13. view on PubMed
Krieger MR, Lee SCS, Fu T, Cornejo MC, He KL, Howe R, Saldivar V, Zhang AL, Chanfreau GF, Teitell MA, Xiao X, Shackelford DB, Koehler CM. Characterization of Mitochondrial Double-Stranded RNA Levels in Non-Small Cell Lung Carcinoma. Cancer Res Commun. 2026 Apr 01; 6(4):769-782. view on PubMed
Higginson LA, Wang X, He K, Torstrick M, Kim M, Wijeratne HRS, Runnebohm AM, Benayoun BA, MacLean A, Chanfreau GF, Morton DJ. The RNA exosome maintains cellular RNA homeostasis by controlling transcript abundance in the brain. Cell Rep. 2026 Jan 27; 45(1):116729. view on PubMed
Barr K, He KL, Krumbein AJ, Chanfreau GF. Transcription termination promotes splicing efficiency and fidelity in a compact genome. Proc Natl Acad Sci U S A. 2025 Aug 12; 122(32):e2507187122. view on PubMed
Barr K, He KL, Krumbein AJ, Chanfreau GF. Transcription termination promotes splicing efficiency and fidelity in a compact genome. bioRxiv. 2025 Jun 22. view on PubMed
He K, Chanfreau GF. Detection of Nuclear RNA Decay Intermediates Using a Modified Oxford Nanopore RNA Sequencing Strategy. Methods Mol Biol. 2025; 2863:339-358. view on PubMed
Higginson LA, Wang X, He K, Torstrick M, Kim M, Benayoun BA, MacLean A, Chanfreau GF, Morton DJ. The RNA exosome maintains cellular RNA homeostasis by controlling transcript abundance in the brain. bioRxiv. 2024 Oct 30. view on PubMed
Novales NA, Feustel KJ, He KL, Chanfreau GF, Clarke CF. Nonfunctional coq10 mutants maintain the ERMES complex and reveal true phenotypes associated with the loss of the coenzyme Q chaperone protein Coq10. J Biol Chem. 2024 11; 300(11):107820. view on PubMed
Carrocci TJ, DeMario S, He K, Zeps NJ, Harkner CT, Chanfreau GF, Hoskins AA. Functional analysis of the zinc finger modules of the Saccharomyces cerevisiae splicing factor Luc7. RNA. 2024 07 16; 30(8):1058-1069. view on PubMed
Krieger MR, Abrahamian M, He KL, Atamdede S, Hakimjavadi H, Momcilovic M, Ostrow D, Maggo SD, Tsang YP, Gai X, Chanfreau GF, Shackelford DB, Teitell MA, Koehler CM. Trafficking of mitochondrial double-stranded RNA from mitochondria to the cytosol. Life Sci Alliance. 2024 09; 7(9). view on PubMed
Carrocci TJ, DeMario S, He K, Zeps NJ, Harkner CT, Chanfreau G, Hoskins AA. Functional Analysis of the Zinc Finger Modules of the S. cerevisiae Splicing Factor Luc7. bioRxiv. 2024 Feb 04. view on PubMed
Bremer KV, Wu C, Patel AA, He KL, Grunfeld AM, Chanfreau GF, Quinlan ME. Formin tails act as a switch, inhibiting or enhancing processive actin elongation. J Biol Chem. 2024 01; 300(1):105557. view on PubMed
Roy KR, Gabunilas J, Neutel D, Ai M, Yeh Z, Samson J, Lyu G, Chanfreau GF. Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites. Nucleic Acids Res. 2023 12 11; 51(22):12428-12442. view on PubMed
DeMario S, Xu K, He K, Chanfreau GF. Nanoblot: an R-package for visualization of RNA isoforms from long-read RNA-sequencing data. RNA. 2023 08; 29(8):1099-1107. view on PubMed
Roy KR, Gabunilas J, Neutel D, Ai M, Samson J, Lyu G, Chanfreau GF. Spliceosomal mutations decouple 3' splice site fidelity from cellular fitness. bioRxiv. 2023 Jan 12. view on PubMed
Liu Y, DeMario S, He K, Gibbs MR, Barr KW, Chanfreau GF. Splicing inactivation generates hybrid mRNA-snoRNA transcripts targeted by cytoplasmic RNA decay. Proc Natl Acad Sci U S A. 2022 08 02; 119(31):e2202473119. view on PubMed
Gibbs MR, Chanfreau GF. UPF1 adds an m6A feather to its (de)cap. Cell Rep. 2022 05 24; 39(8):110898. view on PubMed
Wang C, Barr K, Neutel D, Roy K, Liu Y, Chanfreau GF. Stress-induced inhibition of mRNA export triggers RNase III-mediated decay of the BDF2 mRNA. RNA. 2021 12; 27(12):1545-1556. view on PubMed
Weathers I, Gabunilas J, Samson J, Roy K, Chanfreau GF. Protocol for High-Resolution Mapping of Splicing Products and Isoforms by RT-PCR Using Fluorescently Labeled Primers. STAR Protoc. 2020 12 18; 1(3):100140. view on PubMed
Wang C, Liu Y, DeMario SM, Mandric I, Gonzalez-Figueroa C, Chanfreau GF. Rrp6 Moonlights in an RNA Exosome-Independent Manner to Promote Cell Survival and Gene Expression during Stress. Cell Rep. 2020 06 09; 31(10):107754. view on PubMed
White JT, Cato T, Deramchi N, Gabunilas J, Roy KR, Wang C, Chanfreau GF, Clarke SG. Protein Methylation and Translation: Role of Lysine Modification on the Function of Yeast Elongation Factor 1A. Biochemistry. 2019 12 10; 58(49):4997-5010. view on PubMed
Roy KR, Chanfreau GF. Robust mapping of polyadenylated and non-polyadenylated RNA 3' ends at nucleotide resolution by 3'-end sequencing. Methods. 2020 04 01; 176:4-13. view on PubMed
Roy K, Chanfreau GF. A global function for transcription factors in assisting RNA polymerase II termination. Transcription. 2018; 9(1):41-46. view on PubMed
Chanfreau GF. Impact of RNA Modifications and RNA-Modifying Enzymes on Eukaryotic Ribonucleases. Enzymes. 2017; 41:299-329. view on PubMed
Gillespie A, Gabunilas J, Jen JC, Chanfreau GF. Mutations of EXOSC3/Rrp40p associated with neurological diseases impact ribosomal RNA processing functions of the exosome in S. cerevisiae. RNA. 2017 04; 23(4):466-472. view on PubMed
Chanfreau GF. Preface. Enzymes. 2017; 41:xi. view on PubMed
Roy K, Gabunilas J, Gillespie A, Ngo D, Chanfreau GF. Common genomic elements promote transcriptional and DNA replication roadblocks. Genome Res. 2016 10; 26(10):1363-1375. view on PubMed
Gabunilas J, Chanfreau G. Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae. PLoS Genet. 2016 Apr; 12(4):e1005999. view on PubMed
Hodko D, Ward T, Chanfreau G. The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases. RNA. 2016 Apr; 22(4):559-70. view on PubMed
Al-Hadid Q, Roy K, Chanfreau G, Clarke SG. Methylation of yeast ribosomal protein Rpl3 promotes translational elongation fidelity. RNA. 2016 Apr; 22(4):489-98. view on PubMed
Kaweeteerawat C, Chang CH, Roy KR, Liu R, Li R, Toso D, Fischer H, Ivask A, Ji Z, Zink JI, Zhou ZH, Chanfreau GF, Telesca D, Cohen Y, Holden PA, Nel AE, Godwin HA. Cu Nanoparticles Have Different Impacts in Escherichia coli and Lactobacillus brevis than Their Microsized and Ionic Analogues. ACS Nano. 2015 Jul 28; 9(7):7215-25. view on PubMed
Chanfreau G. Two degrading decades for RNA. RNA. 2015 Apr; 21(4):584-5. view on PubMed
Roy K, Chanfreau G. Stress-induced nuclear RNA degradation pathways regulate yeast bromodomain factor 2 to promote cell survival. PLoS Genet. 2014 Sep; 10(9):e1004661. view on PubMed
Dzialo MC, Travaglini KJ, Shen S, Roy K, Chanfreau GF, Loo JA, Clarke SG. Translational roles of elongation factor 2 protein lysine methylation. J Biol Chem. 2014 Oct 31; 289(44):30511-30524. view on PubMed
Al-Hadid Q, Roy K, Munroe W, Dzialo MC, Chanfreau GF, Clarke SG. Histidine methylation of yeast ribosomal protein Rpl3p is required for proper 60S subunit assembly. Mol Cell Biol. 2014 Aug; 34(15):2903-16. view on PubMed
Kawashima T, Douglass S, Gabunilas J, Pellegrini M, Chanfreau GF. Widespread use of non-productive alternative splice sites in Saccharomyces cerevisiae. PLoS Genet. 2014 Apr; 10(4):e1004249. view on PubMed
Chanfreau GF. Zinc’ing down RNA polymerase I. Transcription. 2013 Sep-Dec; 4(5):217-20. view on PubMed
Lee YJ, Lee CY, Grzechnik A, Gonzales-Zubiate F, Vashisht AA, Lee A, Wohlschlegel J, Chanfreau GF. RNA polymerase I stability couples cellular growth to metal availability. Mol Cell. 2013 Jul 11; 51(1):105-15. view on PubMed
Hartman E, Wang Z, Zhang Q, Roy K, Chanfreau G, Feigon J. Intrinsic dynamics of an extended hydrophobic core in the S. cerevisiae RNase III dsRBD contributes to recognition of specific RNA binding sites. J Mol Biol. 2013 Feb 08; 425(3):546-62. view on PubMed
Chanfreau G, Tamanoi F. Preface. Enzymes. 2012; 31:x-xii. view on PubMed
Roy K, Chanfreau GF. The Diverse Functions of Fungal RNase III Enzymes in RNA Metabolism. Enzymes. 2012; 31:213-35. view on PubMed
Lee YJ, Huang X, Kropat J, Henras A, Merchant SS, Dickson RC, Chanfreau GF. Sphingolipid signaling mediates iron toxicity. Cell Metab. 2012 Jul 03; 16(1):90-6. view on PubMed
Sayani S, Chanfreau GF. Sequential RNA degradation pathways provide a fail-safe mechanism to limit the accumulation of unspliced transcripts in Saccharomyces cerevisiae. RNA. 2012 Aug; 18(8):1563-72. view on PubMed
Egecioglu DE, Kawashima TR, Chanfreau GF. Quality control of MATa1 splicing and exon skipping by nuclear RNA degradation. Nucleic Acids Res. 2012 Feb; 40(4):1787-96. view on PubMed
Wang Z, Hartman E, Roy K, Chanfreau G, Feigon J. Structure of a yeast RNase III dsRBD complex with a noncanonical RNA substrate provides new insights into binding specificity of dsRBDs. Structure. 2011 Jul 13; 19(7):999-1010. view on PubMed
Toesca I, Nery CR, Fernandez CF, Sayani S, Chanfreau GF. Cryptic transcription mediates repression of subtelomeric metal homeostasis genes. PLoS Genet. 2011 Jun; 7(6):e1002163. view on PubMed
Koo BK, Park CJ, Fernandez CF, Chim N, Ding Y, Chanfreau G, Feigon J. Structure of H/ACA RNP protein Nhp2p reveals cis/trans isomerization of a conserved proline at the RNA and Nop10 binding interface. J Mol Biol. 2011 Sep 02; 411(5):927-42. view on PubMed
Egecioglu DE, Chanfreau G. Proofreading and spellchecking: a two-tier strategy for pre-mRNA splicing quality control. RNA. 2011 Mar; 17(3):383-9. view on PubMed
Chanfreau GF. A dual role for RNA splicing signals. EMBO Rep. 2010 Oct; 11(10):720-1. view on PubMed
Chanfreau G. InvIGOrating mRNAs during Quiescence. Mol Cell. 2010 May 14; 38(3):317-8. view on PubMed
Kawashima T, Pellegrini M, Chanfreau GF. Nonsense-mediated mRNA decay mutes the splicing defects of spliceosome component mutations. RNA. 2009 Dec; 15(12):2236-47. view on PubMed
Singh M, Gonzales FA, Cascio D, Heckmann N, Chanfreau G, Feigon J. Structure and functional studies of the CS domain of the essential H/ACA ribonucleoparticle assembly protein SHQ1. J Biol Chem. 2009 Jan 16; 284(3):1906-16. view on PubMed
Sayani S, Janis M, Lee CY, Toesca I, Chanfreau GF. Widespread impact of nonsense-mediated mRNA decay on the yeast intronome. Mol Cell. 2008 Aug 08; 31(3):360-70. view on PubMed
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Maréchal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fernández E, Fukuzawa H, González-Ballester D, González-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Riaño-Pachón DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Martínez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science. 2007 Oct 12; 318(5848):245-50. view on PubMed
Oberstrass FC, Lee A, Stefl R, Janis M, Chanfreau G, Allain FH. Shape-specific recognition in the structure of the Vts1p SAM domain with RNA. Nat Struct Mol Biol. 2006 Feb; 13(2):160-7. view on PubMed
Egecioglu DE, Henras AK, Chanfreau GF. Contributions of Trf4p- and Trf5p-dependent polyadenylation to the processing and degradative functions of the yeast nuclear exosome. RNA. 2006 Jan; 12(1):26-32. view on PubMed
Chanfreau GF. CUTting genetic noise by polyadenylation-induced RNA degradation. Trends Cell Biol. 2005 Dec; 15(12):635-7. view on PubMed
Lee A, Henras AK, Chanfreau G. Multiple RNA surveillance pathways limit aberrant expression of iron uptake mRNAs and prevent iron toxicity in S. cerevisiae. Mol Cell. 2005 Jul 01; 19(1):39-51. view on PubMed
Henras AK, Sam M, Hiley SL, Wu H, Hughes TR, Feigon J, Chanfreau GF. Biochemical and genomic analysis of substrate recognition by the double-stranded RNA binding domain of yeast RNase III. RNA. 2005 Aug; 11(8):1225-37. view on PubMed
Zer C, Chanfreau G. Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradation. J Biol Chem. 2005 Aug 12; 280(32):28997-9003. view on PubMed
Yang PK, Hoareau C, Froment C, Monsarrat B, Henry Y, Chanfreau G. Cotranscriptional recruitment of the pseudouridylsynthetase Cbf5p and of the RNA binding protein Naf1p during H/ACA snoRNP assembly. Mol Cell Biol. 2005 Apr; 25(8):3295-304. view on PubMed
Sam M, Henras AK, Chanfreau G. A conserved major groove antideterminant for Saccharomyces cerevisiae RNase III recognition. Biochemistry. 2005 Mar 22; 44(11):4181-7. view on PubMed
Sam M, Hwang JH, Chanfreau G, Abu-Omar MM. Hydroxyl radical is the active species in photochemical DNA strand scission by bis(peroxo)vanadium(V) phenanthroline. Inorg Chem. 2004 Dec 27; 43(26):8447-55. view on PubMed
Henras AK, Bertrand E, Chanfreau G. A cotranscriptional model for 3'-end processing of the Saccharomyces cerevisiae pre-ribosomal RNA precursor. RNA. 2004 Oct; 10(10):1572-85. view on PubMed
Wu H, Henras A, Chanfreau G, Feigon J. Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase III. Proc Natl Acad Sci U S A. 2004 Jun 01; 101(22):8307-12. view on PubMed
Lee CY, Lee A, Chanfreau G. The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5'-end processing of S. cerevisiae box C/D snoRNAs. RNA. 2003 Nov; 9(11):1362-70. view on PubMed
Chanfreau G. Conservation of RNase III processing pathways and specificity in hemiascomycetes. Eukaryot Cell. 2003 Oct; 2(5):901-9. view on PubMed
Danin-Kreiselman M, Lee CY, Chanfreau G. RNAse III-mediated degradation of unspliced pre-mRNAs and lariat introns. Mol Cell. 2003 May; 11(5):1279-89. view on PubMed
Yang PK, Rotondo G, Porras T, Legrain P, Chanfreau G. The Shq1p.Naf1p complex is required for box H/ACA small nucleolar ribonucleoprotein particle biogenesis. J Biol Chem. 2002 Nov 22; 277(47):45235-42. view on PubMed
Wu H, Yang PK, Butcher SE, Kang S, Chanfreau G, Feigon J. A novel family of RNA tetraloop structure forms the recognition site for Saccharomyces cerevisiae RNase III. EMBO J. 2001 Dec 17; 20(24):7240-9. view on PubMed
Kufel J, Allmang C, Chanfreau G, Petfalski E, Lafontaine DL, Tollervey D. Precursors to the U3 small nucleolar RNA lack small nucleolar RNP proteins but are stabilized by La binding. Mol Cell Biol. 2000 Aug; 20(15):5415-24. view on PubMed
Chanfreau G, Buckle M, Jacquier A. Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III. Proc Natl Acad Sci U S A. 2000 Mar 28; 97(7):3142-7. view on PubMed
Allmang C, Kufel J, Chanfreau G, Mitchell P, Petfalski E, Tollervey D. Functions of the exosome in rRNA, snoRNA and snRNA synthesis. EMBO J. 1999 Oct 01; 18(19):5399-410. view on PubMed
Chanfreau G, Gouyette C, Schwer B, Jacquier A. Trans-complementation of the second step of pre-mRNA splicing by exogenous 5' exons. RNA. 1999 Jul; 5(7):876-82. view on PubMed
Chanfreau G, Legrain P, Jacquier A. Yeast RNase III as a key processing enzyme in small nucleolar RNAs metabolism. J Mol Biol. 1998 Dec 11; 284(4):975-88. view on PubMed
Chanfreau G, Rotondo G, Legrain P, Jacquier A. Processing of a dicistronic small nucleolar RNA precursor by the RNA endonuclease Rnt1. EMBO J. 1998 Jul 01; 17(13):3726-37. view on PubMed
Nolte A, Chanfreau G, Jacquier A. Influence of substrate structure on in vitro ribozyme activity of a group II intron. RNA. 1998 Jun; 4(6):694-708. view on PubMed
Chanfreau G, Elela SA, Ares M, Guthrie C. Alternative 3'-end processing of U5 snRNA by RNase III. Genes Dev. 1997 Oct 15; 11(20):2741-51. view on PubMed
Chanfreau G, Noble SM, Guthrie C. Essential yeast protein with unexpected similarity to subunits of mammalian cleavage and polyadenylation specificity factor (CPSF). Science. 1996 Nov 29; 274(5292):1511-4. view on PubMed
Chanfreau G, Jacquier A. An RNA conformational change between the two chemical steps of group II self-splicing. EMBO J. 1996 Jul 01; 15(13):3466-76. view on PubMed
Chanfreau G, Jacquier A. Catalytic site components common to both splicing steps of a group II intron. Science. 1994 Nov 25; 266(5189):1383-7. view on PubMed
Chanfreau G, Legrain P, Dujon B, Jacquier A. Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S. cerevisiae. Nucleic Acids Res. 1994 Jun 11; 22(11):1981-7. view on PubMed
Chanfreau G, Jacquier A. Interaction of intronic boundaries is required for the second splicing step efficiency of a group II intron. EMBO J. 1993 Dec 15; 12(13):5173-80. view on PubMed
