David Wong

1. My laboratory spearheads the use of genome-wide and proteome-wide high-throughput technologies to harness the diagnostic and biological determinants pertaining to the pathogenesis of oral/head and neck cancer. In particular we are focusing on the genomic and proteomic determinants of oral cancer progression. Using a patient-based genome/proteome-wide approach; we are harnessing the genomic and proteomic determinants that distinguish progressing from non-progression oral precancers. For genomic defects, a concurrent approach to identify gene copy number abnormalities (CNA) by cDNA microarray comparative genomic hybridization (CGH) as well as SNP-based loss of heterozygosity (LOH) using the Affymetrix 100K SNP-based Mapping Arrays. For expression analysis, the Affymetrix 133+ 2 and all exon high-density oligonucleotide arrays are used. For proteomic analysis, LC-MS/MS as well as single cell proteomics are creatively being integrated. A key component to a systems approach (Weighted-Gene Co-Expression Network Analysis, WGCNA) is to seek out molecular concordance of detectable molecular defects. a. Das S, Ansel KM, Bitzer M, Breakefield XO, Charest A, Galas DJ, Gerstein MB, Gupta M, Milosavljevic A, McManus MT, Patel T, Raffai RL, Rozowsky J, Roth ME, Saugstad JA, Van Keuren-Jensen K, Weaver AM, Laurent LC. The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research. Cell. 2019 Apr 4;177(2):231-242. PubMed Central PMCID: PMC6601620. b. Yu T, Ye H, Sun W, Li KC, Chen Z, Jacobs S, Bailey DK, Wong DT, Zhou X. A forward-backward fragment assembling algorithm for the identification of genomic amplification and deletion breakpoints using high-density single nucleotide polymorphism (SNP) array. BMC Bioinformatics. 2007 May 3;8:145. PubMed Central PMCID: PMC1868765. c. Zhou X, Temam S, Chen Z, Ye H, Mao L, Wong DT. Allelic imbalance analysis of oral tongue squamous cell carcinoma by high-density single nucleotide polymorphism arrays using whole-genome amplified DNA. Hum Genet. 2005 Dec;118(3-4):504-7. PubMed PMID: 16193324. d. Zhou X, Mok SC, Chen Z, Li Y, Wong DT. Concurrent analysis of loss of heterozygosity (LOH) and copy number abnormality (CNA) for oral premalignancy progression using the Affymetrix 10K SNP mapping array. Hum Genet. 2004 Sep;115(4):327-30. PubMed PMID: 15290239. 2. We subscribe to the belief that a central defect in human cancer lies in the dysregulation of cell cycle control. Establishing a molecular database of cell cycle regulatory genes dysregulated in head and neck cancer and understanding the molecular and biochemical pathways of these genes are areas of prime interest. Using differential expression screening assays we have identified a number of cellular genes that are differentially expressed in squamous cell carcinoma. CDK2-AP1 is a differentially expressed cellular gene that is currently being studied. CDK2-AP1 is a novel gene exhibiting growth suppressor properties. CDK2-AP1 exerts its growth suppressor function by negatively regulating the activities of DNA polymerase- /primase and cyclin-dependent kinase 2 (CDK2). CDK2-AP1 associates with DNA polymerase-/primase suppressing DNA replication, affecting predominantly the initiation step. CDK2-AP1 has been found to associates with the monomeric non-phosphorylated form of CDK2, suppressing CDK2-associated kinase activities and cell cycle progression. CDK2-AP1 also targets CDK2 for proteolysis. We are investigating the detailed biochemical and genetic mechanisms of CDK2-AP1 in cell cycle regulation, normal development and carcinogenesis. The recent excitement of this research includes finding that CDK2-AP1 -nulled mice are embryonically lethal and that CDK2-AP1 is an epigenetic regulator for differentiation competency for mouse embryonic stem cells, placing this gene at a pivotal juncture of stem cell differentiation and development. a. Kim JJ, Khalid O, Vo S, Sun HH, Wong DT, Kim Y. A novel regulatory factor recruits the nucleosome remodeling complex to wingless integrated (Wnt) signaling gene promoters in mouse embryonic stem cells. J Biol Chem. 2012 Nov 30;287(49):41103-17. PubMed Central PMCID: PMC3510811. b. Kim Y, Deshpande A, Dai Y, Kim JJ, Lindgren A, Conway A, Clark AT, Wong DT. Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. J Biol Chem. 2009 Aug 28;284(35):23405-14. PubMed Central PMCID: PMC2749114. c. Deshpande AM, Dai YS, Kim Y, Kim J, Kimlin L, Gao K, Wong DT. Cdk2ap1 is required for epigenetic silencing of Oct4 during murine embryonic stem cell differentiation. J Biol Chem. 2009 Mar 6;284(10):6043-7. PubMed Central PMCID: PMC2649091. d. Shintani S, Ohyama H, Zhang X, McBride J, Matsuo K, Tsuji T, Hu MG, Hu G, Kohno Y, Lerman M, Todd R, Wong DT. p12(DOC-1) is a novel cyclin-dependent kinase 2-associated protein. Mol Cell Biol. 2000 Sep;20(17):6300-7. PubMed Central PMCID: PMC86104. 3. The early detection of cancer by non-invasive means has been the holy grail for cancer researchers. My research group has been spearheading a series of concerted efforts to spearhead the scientific and translational frontiers of salivary diagnostics. The use of saliva, a non-invasive biofluid, for oral and systemic disease detection, for disease progression, and for therapeutic monitoring are highly desirable goals. We have harnessed and defined five diagnostic alphabets from human saliva: salivary proteome, transcriptome, micro-RNA, metabolome and microbiome. The availability of these diagnostic alphabets greatly enhanced the translational utilities of saliva. In parallel to the biomarker efforts, point-of-care technologies are being developed for clinical applications. The “Oral Fluid NanoSensor Test (OFNASET)” is a prototype nanotechnology-based point-of-care sensor that will have the ability to detect multiplex analytes in saliva for disease detection. Two additional highly impactful outcomes emerged from these initiatives. One is the discovery of salivary extracellular RNA in saliva, including a previously unnoticed landscape of non-coding RNA. This project by the NIH Common Fund on “Extracellular RNA Communication Consortium” opened a new horizon of saliva biology. A second facet of excitement is the finding that saliva is an optimal biofluid for liquid biopsy (saliva liquid biopsy, sLB) for detection of actionable mutations in human cancers. Using an electrochemical technology called electric field-induced release and measurement (EFIRM), circulating tumor DNA (ctDNA) was detectable in lung cancer patients with near perfect concordance with tissue based biopsy genotyping. a. Murillo OD, Thistlethwaite W, Rozowsky J, Subramanian SL, Lucero R, Shah N, Jackson AR, Srinivasan S, Chung A, Laurent CD, Kitchen RR, Galeev T, Warrell J, Diao JA, Welsh JA, Hanspers K, Riutta A, Burgstaller-Muehlbacher S, Shah RV, Yeri A, Jenkins LM, Ahsen ME, Cordon-Cardo C, Dogra N, Gifford SM, Smith JT, Stolovitzky G, Tewari AK, Wunsch BH, Yadav KK, Danielson KM, Filant J, Moeller C, Nejad P, Paul A, Simonson B, Wong DK, Zhang X, Balaj L, Gandhi R, Sood AK, Alexander RP, Wang L, Wu C, Wong DTW, Galas DJ, Van Keuren-Jensen K, Patel T, Jones JC, Das S, Cheung KH, Pico AR, Su AI, Raffai RL, Laurent LC, Roth ME, Gerstein MB, Milosavljevic A. exRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types and Their Carriers Present across Human Biofluids. Cell. 2019 Apr 4;177(2):463-477.e15. PubMed Central PMCID: PMC6616370. b. Wei F, Lin CC, Joon A, Feng Z, Troche G, Lira ME, Chia D, Mao M, Ho CL, Su WC, Wong DT. Noninvasive saliva-based EGFR gene mutation detection in patients with lung cancer. Am J Respir Crit Care Med. 2014 Nov 15;190(10):1117-26. PubMed Central PMCID: PMC5447327. c. Li Y, Elashoff D, Oh M, Sinha U, St John MA, Zhou X, Abemayor E, Wong DT. Serum circulating human mRNA profiling and its utility for oral cancer detection. J Clin Oncol. 2006 Apr 10;24(11):1754-60. PubMed PMID: 16505414. d. Li Y, St John MA, Zhou X, Kim Y, Sinha U, Jordan RC, Eisele D, Abemayor E, Elashoff D, Park NH, Wong DT. Salivary transcriptome diagnostics for oral cancer detection. Clin Cancer Res. 2004 Dec 15;10(24):8442-50. PubMed PMID: 15623624.

Websites:

W Lab

Publications:

Tran HL, Zheng W, Issadore DA, Im H, Cho YK, Zhang Y, Liu D, Liu Y, Li B, Liu F, Wong DTW, Sun J, Qian K, He M, Wan M, Zeng Y, Cheng K, Huang TJ, Chiu DT, Lee LP, Zheng L, Godwin AK, Kalluri R, Soper SA, Hu TY. Extracellular Vesicles for Clinical Diagnostics: From Bulk Measurements to Single-Vesicle Analysis. ACS Nano. 2025 Aug 12; 19(31):28021-28109. view on PubMed

Qian J, Xia J, Chiang S, Liu JF, Li K, Li F, Wei F, Aziz M, Kim Y, Go V, Morizio J, Zhong R, He Y, Yang K, Yang OO, Wong DTW, Lee LP, Huang TJ. Rapid and comprehensive detection of viral antibodies and nucleic acids via an acoustofluidic integrated molecular diagnostics chip: AIMDx. Sci Adv. 2025 Jan 17; 11(3):eadt5464. view on PubMed

Mohammadi A, Chiang S, Li F, Wei F, Lau CS, Aziz M, Ibarrondo FJ, Fulcher JA, Yang OO, Chia D, Kim Y, Wong DTW. Direct detection of 4-dimensions of SARS-CoV-2: infection (vRNA), infectivity (antigen), binding antibody, and functional neutralizing antibody in saliva. Sci Rep. 2024 12 28; 14(1):30792. view on PubMed

Nordham KD, Tatum D, Attia AS, Patel MB, Paramesh A, Duchesne JC, Nahmias J, Maiga AW, Peetz AB, Udekwu PO, Stiles A, Shell C, Stodghill JD, Maghsoudi T, Iacullo E, McLafferty B, Coonan E, Boudreau RM, Zimmerman SA, Shammassian B, Egodage T, Aramento I, Morris P, Metheny J, Farrell MS, Painter MD, McCabe OT, Spadafore P, Wong DT, Serrano J, Sciarretta JD, Kim P, Hayton R, Gonzales D, Murry J, Meadows K, Jacobson LE, Williams JM, Bernard AC, Smith B, Morrissey SL, Patel N, Tabello D, Teicher E, Chowdhury SM, Ahmad F, Marcos BS, West MA, Jacome TH, Davis G, Marks JA, Rattigan D, Haan JM, Lightwine K, Matsushima K, Park S, Santos A, Shrestha K, Sawyer R, VandenBerg S, Jean RJ, Hicks RC, Lueckel S, Bugaev N, Abosena W, Alvarez C, Lieser MJ, McDonald H, Dumas RP, Fitzgerald CA, Terzian WTH, Tian Y, Mousafeiris V, Mulita F, Berne JD, Mederos DR, Smith AA, Taghavi S. Impact of catastrophic brain injury guidelines on organ donation rates: Results of an EAST multicenter trial. J Trauma Acute Care Surg. 2025 Jan 01; 98(1):117-126. view on PubMed

Haslam DE, Liang L, Guo K, Martínez-Lozano M, Pérez CM, Lee CH, Morou-Bermudez E, Clish C, Wong DTW, Manson JE, Hu FB, Stampfer MJ, Joshipura K, Bhupathiraju SN. Correction: Discovery and validation of plasma, saliva and multi-fluid plasma-saliva metabolomic scores predicting insulin resistance and diabetes progression or regression among Puerto Rican adults. Diabetologia. 2024 Sep; 67(9):2016. view on PubMed

Kamounah S, Wei F, Park JK, Song YW, Chia D, Wong DTW, Pedersen AML. Corrigendum to paper entitled "Seronegative patients with primary Sjogren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva" [Published in: BBA - Mol. Basis Dis. Volume 1870, Issue 5, June 2024, 167168]. Biochim Biophys Acta Mol Basis Dis. 2024 Dec; 1870(8):167474. view on PubMed

Khanani AM, Sadda SR, Sarraf D, Tadayoni R, Wong DT, Kempf AS, Saffar I, Gedif K, Chang A. Effect of Brolucizumab and Aflibercept on the Maximum Thickness of Pigment Epithelial Detachments and Sub-Retinal Pigment Epithelium Fluid in HAWK and HARRIER. Ophthalmol Retina. 2025 Jan; 9(1):13-21. view on PubMed

Cheng JC, Swarup N, Morselli M, Huang WL, Aziz M, Caggiano C, Kordi M, Patel AA, Chia D, Kim Y, Li F, Wei F, Zaitlen N, Krysan K, Dubinett S, Pellegrini M, Wong DTW. Single-stranded pre-methylated 5mC adapters uncover the methylation profile of plasma ultrashort Single-stranded cell-free DNA. Nucleic Acids Res. 2024 Jun 24; 52(11):e50. view on PubMed

Haslam DE, Liang L, Guo K, Martínez-Lozano M, Pérez CM, Lee CH, Morou-Bermudez E, Clish C, Wong DTW, Manson JE, Hu FB, Stampfer MJ, Joshipura K, Bhupathiraju SN. Discovery and validation of plasma, saliva and multi-fluid plasma-saliva metabolomic scores predicting insulin resistance and diabetes progression or regression among Puerto Rican adults. Diabetologia. 2024 Sep; 67(9):1838-1852. view on PubMed

Kamounah S, Wei F, Park JK, Song YW, Chia D, Wong DTW, Pedersen AML. Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. Biochim Biophys Acta Mol Basis Dis. 2024 06; 1870(5):167168. view on PubMed

Chiang S, Grogan T, Kamounah S, Wei F, Tayob N, Kim JY, Kyun Park J, Akin D, Elashoff DA, Pedersen AML, Song YW, Wong DTW, Chia D. Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca. RMD Open. 2024 04 10; 10(2). view on PubMed

Cheng JC, Swarup N, Wong DTW, Chia D. A review on the impact of single-stranded library preparation on plasma cell-free diversity for cancer detection. Front Oncol. 2024; 14:1332004. view on PubMed

Mohammadi A, Chiang S, Li F, Wei F, Lau CS, Aziz M, Ibarrondo FJ, Fulcher JA, Yang OO, Chia D, Kim Y, Wong DTW. Direct Detection of 4-Dimensions of SARS-CoV-2: Infection (vRNA), Infectivity (Antigen), Binding Antibody, and Functional Neutralizing Antibody in Saliva. Res Sq. 2023 Dec 28. view on PubMed

Radaic A, Kamarajan P, Cho A, Wang S, Hung GC, Najarzadegan F, Wong DT, Ton-That H, Wang CY, Kapila YL. Biological biomarkers of oral cancer. Periodontol 2000. 2024 Oct; 96(1):250-280. view on PubMed

Swarup N, Cheng J, Choi I, Heo YJ, Kordi M, Aziz M, Arora A, Li F, Chia D, Wei F, Elashoff D, Zhang L, Kim S, Kim Y, Wong DTW. Correction: Multi-faceted attributes of salivary cell-free DNA as liquid biopsy biomarkers for gastric cancer detection. Biomark Res. 2023 Nov 09; 11(1):96. view on PubMed

Cheng J, Swarup N, Li F, Kordi M, Lin CC, Yang SC, Huang WL, Aziz M, Kim Y, Chia D, Yeh YM, Wei F, Zheng D, Zhang L, Pellegrini M, Su WC, Wong DTW. Distinct Features of Plasma Ultrashort Single-Stranded Cell-Free DNA as Biomarkers for Lung Cancer Detection. Clin Chem. 2023 11 02; 69(11):1270-1282. view on PubMed

Swarup N, Cheng J, Choi I, Heo YJ, Kordi M, Aziz M, Arora A, Li F, Chia D, Wei F, Elashoff D, Zhang L, Kim S, Kim Y, Wong DTW. Multi-faceted attributes of salivary cell-free DNA as liquid biopsy biomarkers for gastric cancer detection. Biomark Res. 2023 Oct 10; 11(1):90. view on PubMed

Batool SM, Yekula A, Khanna P, Hsia T, Gamblin AS, Ekanayake E, Escobedo AK, You DG, Castro CM, Im H, Kilic T, Garlin MA, Skog J, Dinulescu DM, Dudley J, Agrawal N, Cheng J, Abtin F, Aberle DR, Chia D, Elashoff D, Grognan T, Krysan K, Oh SS, Strom C, Tu M, Wei F, Xian RR, Skates SJ, Zhang DY, Trinh T, Watson M, Aft R, Rawal S, Agarwal A, Kesmodel SB, Yang C, Shen C, Hochberg FH, Wong DTW, Patel AA, Papadopoulos N, Bettegowda C, Cote RJ, Srivastava S, Lee H, Carter BS, Balaj L. The Liquid Biopsy Consortium: Challenges and opportunities for early cancer detection and monitoring. Cell Rep Med. 2023 10 17; 4(10):101198. view on PubMed

Swarup N, Cheng J, Choi I, Heo YJ, Kordi M, Li F, Aziz M, Chia D, Wei F, Elashoff D, Zhang L, Kim S, Kim Y, Wong DTW. Multi-Faceted Attributes of Salivary Cell-free DNA as Liquid Biopsy Biomarkers for Gastric Cancer Detection. Res Sq. 2023 Jul 14. view on PubMed

Kamounah S, Tayob N, Chiang S, Wei F, Park JK, Kwon HM, Feng Z, Chia D, Pedersen AML, Song YW, Wong DTW. Immunoassay Detects Salivary Anti-SSA/Ro-52 Autoantibodies in Seronegative Patients with Primary Sjögren's Syndrome. Immunohorizons. 2023 07 01; 7(7):554-561. view on PubMed

Wei F, Yu P, Cheng J, Li F, Chia D, Wong DTW. Single-Droplet Microsensor for Ultra-Short Circulating EFGR Mutation Detection in Lung Cancer Based on Multiplex EFIRM Liquid Biopsy. Int J Mol Sci. 2023 Jun 20; 24(12). view on PubMed

Salfer B, Havo D, Kuppinger S, Wong DTW, Li F, Zhang L. Evaluating Pre-Analytical Variables for Saliva Cell-Free DNA Liquid Biopsy. Diagnostics (Basel). 2023 May 09; 13(10). view on PubMed

Bansal A, Hamli H, Lee WW, Sarraf D, Sadda S, Berger AR, Wong DT, Kertes PJ, Kohly RP, Hillier RJ, Muni RH. En Face OCT in Diagnosis of Persistent Subretinal Fluid and Outer Retinal Folds after Rhegmatogenous Retinal Detachment Repair. Ophthalmol Retina. 2023 06; 7(6):496-502. view on PubMed

Kaczor-Urbanowicz KE, Wong DTW. RNA Sequencing Analysis of Saliva exRNA. Methods Mol Biol. 2023; 2588:3-11. view on PubMed

Zhang J, Chen C, Becker R, Rufo J, Yang S, Mai J, Zhang P, Gu Y, Wang Z, Ma Z, Xia J, Hao N, Tian Z, Wong DTW, Sadovsky Y, Lee LP, Huang TJ. A solution to the biophysical fractionation of extracellular vesicles: Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER). Sci Adv. 2022 Nov 25; 8(47):eade0640. view on PubMed

Liu Z, Li T, Wang Z, Liu J, Huang S, Min BH, An JY, Kim KM, Kim S, Chen Y, Liu H, Kim Y, Wong DTW, Huang TJ, Xie YH. Gold Nanopyramid Arrays for Non-Invasive Surface-Enhanced Raman Spectroscopy-Based Gastric Cancer Detection via sEVs. ACS Appl Nano Mater. 2022 Sep 23; 5(9):12506-12517. view on PubMed

Bansal A, Lee WW, Sarraf D, Sadda SR, Berger AR, Wong DT, Kertes PJ, Kohly RP, Hillier RJ, Muni RH. Persistent subfoveal fluid in pneumatic retinopexy versus pars plana vitrectomy for rhegmatogenous retinal detachment: posthoc analysis of the PIVOT randomised trial. Br J Ophthalmol. 2023 Nov; 107(11):1693-1697. view on PubMed

Kaczor-Urbanowicz KE, Saad M, Grogan TR, Li F, Heo YJ, Elashoff D, Bresalier RS, Wong DTW, Kim Y. Performance of Salivary Extracellular RNA Biomarker Panels for Gastric Cancer Differs between Distinct Populations. Cancers (Basel). 2022 Jul 26; 14(15). view on PubMed

Li F, Wei F, Grogan TR, Elashoff DE, Vu D, Vigerust DJ, Gupta R, Wong DTW. Proficiency Testing of Epidermal Growth Factor Receptor Mutations Detection in Saliva Using Spectrum Saliva Collector (SDNA-1000) and Preservative Solution Detected by Electric Field-Induced Release and Measurement. Biopreserv Biobank. 2022 10; 20(5):461-464. view on PubMed

Mateescu B, Jones JC, Alexander RP, Alsop E, An JY, Asghari M, Boomgarden A, Bouchareychas L, Cayota A, Chang HC, Charest A, Chiu DT, Coffey RJ, Das S, De Hoff P, deMello A, D'Souza-Schorey C, Elashoff D, Eliato KR, Franklin JL, Galas DJ, Gerstein MB, Ghiran IH, Go DB, Gould S, Grogan TR, Higginbotham JN, Hladik F, Huang TJ, Huo X, Hutchins E, Jeppesen DK, Jovanovic-Talisman T, Kim BYS, Kim S, Kim KM, Kim Y, Kitchen RR, Knouse V, LaPlante EL, Lebrilla CB, Lee LJ, Lennon KM, Li G, Li F, Li T, Liu T, Liu Z, Maddox AL, McCarthy K, Meechoovet B, Maniya N, Meng Y, Milosavljevic A, Min BH, Morey A, Ng M, Nolan J, De Oliveira Junior GP, Paulaitis ME, Phu TA, Raffai RL, Reátegui E, Roth ME, Routenberg DA, Rozowsky J, Rufo J, Senapati S, Shachar S, Sharma H, Sood AK, Stavrakis S, Stürchler A, Tewari M, Tosar JP, Tucker-Schwartz AK, Turchinovich A, Valkov N, Van Keuren-Jensen K, Vickers KC, Vojtech L, Vreeland WN, Wang C, Wang K, Wang Z, Welsh JA, Witwer KW, Wong DTW, Xia J, Xie YH, Yang K, Zaborowski MP, Zhang C, Zhang Q, Zivkovic AM, Laurent LC. Phase 2 of extracellular RNA communication consortium charts next-generation approaches for extracellular RNA research. iScience. 2022 Aug 19; 25(8):104653. view on PubMed

Cheng J, Morselli M, Huang WL, Heo YJ, Pinheiro-Ferreira T, Li F, Wei F, Chia D, Kim Y, He HJ, Cole KD, Su WC, Pellegrini M, Wong DTW. Plasma contains ultrashort single-stranded DNA in addition to nucleosomal cell-free DNA. iScience. 2022 Jul 15; 25(7):104554. view on PubMed

Cohn W, Zhu C, Campagna J, Bilousova T, Spilman P, Teter B, Li F, Guo R, Elashoff D, Cole GM, Avidan A, Faull KF, Whitelegge J, Wong DTW, John V. Integrated Multiomics Analysis of Salivary Exosomes to Identify Biomarkers Associated with Changes in Mood States and Fatigue. Int J Mol Sci. 2022 May 09; 23(9). view on PubMed

Munk MR, Kashani AH, Tadayoni R, Korobelnik JF, Wolf S, Pichi F, Koh A, Ishibazawa A, Gaudric A, Loewenstein A, Lumbroso B, Ferrara D, Sarraf D, Wong DT, Skondra D, Rodriguez FJ, Staurenghi G, Pearce I, Kim JE, Freund KB, Parodi MB, Waheed NK, Rosen R, Spaide RF, Nakao S, Sadda S, Vujosevic S, Wong TY, Murata T, Chakravarthy U, Ogura Y, Huf W, Tian M. Recommendations for OCT Angiography Reporting in Retinal Vascular Disease: A Delphi Approach by International Experts. Ophthalmol Retina. 2022 09; 6(9):753-761. view on PubMed

Dinis M, Agnello M, Cen L, Shokeen B, He X, Shi W, Wong DTW, Lux R, Tran NC. Oral Microbiome: Streptococcus mutans/Caries Concordant-Discordant Children. Front Microbiol. 2022; 13:782825. view on PubMed

Figueiredo N, Sarraf D, Gunnemann F, Sadda SR, Bansal A, Berger AR, Wong DT, Kohly RP, Kertes PJ, Hillier RJ, Muni RH. Longitudinal Assessment of Ellipsoid Zone Recovery Using En Face Optical Coherence Tomography After Retinal Detachment Repair. Am J Ophthalmol. 2022 04; 236:212-220. view on PubMed

Lee WW, Bansal A, Sadda SR, Sarraf D, Berger AR, Wong DT, Kertes PJ, Kohly RP, Hillier RJ, Muni RH. Outer Retinal Folds after Pars Plana Vitrectomy vs. Pneumatic Retinopexy for Retinal Detachment Repair: Post hoc analysis from PIVOT. Ophthalmol Retina. 2022 03; 6(3):234-242. view on PubMed

Haslam DE, Liang L, Wang DD, Kelly RS, Wittenbecher C, Pérez CM, Martínez M, Lee CH, Clish CB, Wong DTW, Parnell LD, Lai CQ, Ordovás JM, Manson JE, Hu FB, Stampfer MJ, Tucker KL, Joshipura KJ, Bhupathiraju SN. Associations of network-derived metabolite clusters with prevalent type 2 diabetes among adults of Puerto Rican descent. BMJ Open Diabetes Res Care. 2021 08; 9(1). view on PubMed

Kim C, Xi L, Cultraro CM, Wei F, Jones G, Cheng J, Shafiei A, Pham TH, Roper N, Akoth E, Ghafoor A, Misra V, Monkash N, Strom C, Tu M, Liao W, Chia D, Morris C, Steinberg SM, Bagheri H, Wong DTW, Raffeld M, Guha U. Longitudinal Circulating Tumor DNA Analysis in Blood and Saliva for Prediction of Response to Osimertinib and Disease Progression in EGFR-Mutant Lung Adenocarcinoma. Cancers (Basel). 2021 Jul 03; 13(13). view on PubMed

Chiang SH, Tu M, Cheng J, Wei F, Li F, Chia D, Garner O, Chandrasekaran S, Bender R, Strom CM, Wong DTW. Development and validation of a quantitative, non-invasive, highly sensitive and specific, electrochemical assay for anti-SARS-CoV-2 IgG antibodies in saliva. PLoS One. 2021; 16(7):e0251342. view on PubMed

Muni RH, Felfeli T, Sadda SR, Juncal VR, Francisconi CLM, Nittala MG, Lindenberg S, Gunnemann F, Berger AR, Wong DT, Altomare F, Giavedoni LR, Kohly RP, Kertes PJ, Sarraf D, Hillier RJ. Postoperative Photoreceptor Integrity Following Pneumatic Retinopexy vs Pars Plana Vitrectomy for Retinal Detachment Repair: A Post Hoc Optical Coherence Tomography Analysis From the Pneumatic Retinopexy Versus Vitrectomy for the Management of Primary Rhegmatogenous Retinal Detachment Outcomes Randomized Trial. JAMA Ophthalmol. 2021 Jun 01; 139(6):620-627. view on PubMed

Gu Y, Chen C, Mao Z, Bachman H, Becker R, Rufo J, Wang Z, Zhang P, Mai J, Yang S, Zhang J, Zhao S, Ouyang Y, Wong DTW, Sadovsky Y, Huang TJ. Acoustofluidic centrifuge for nanoparticle enrichment and separation. Sci Adv. 2021 01; 7(1). view on PubMed

Chiang SH, Tu M, Cheng J, Wei F, Li F, Chia D, Garner O, Chandrasekaran S, Bender R, Strom CM, Wong DTW. Development and validation of a highly sensitive and specific electrochemical assay to quantify anti-SARS-CoV-2 IgG antibodies to facilitate pandemic surveillance and monitoring of vaccine response. medRxiv. 2020 Nov 16. view on PubMed

Tavanasefat H, Li F, Koyano K, Gourtani BK, Marty V, Mulpuri Y, Lee SH, Shin KH, Wong DTW, Xiao X, Spigelman I, Kim Y. Molecular consequences of fetal alcohol exposure on amniotic exosomal miRNAs with functional implications for stem cell potency and differentiation. PLoS One. 2020; 15(11):e0242276. view on PubMed

Kaczor-Urbanowicz KE, Cheng J, King JC, Sedarat A, Pandol SJ, Farrell JJ, Wong DTW, Kim Y. Reviews on Current Liquid Biopsy for Detection and Management of Pancreatic Cancers. Pancreas. 2020 10; 49(9):1141-1152. view on PubMed

Li N, Guha U, Kim C, Ye L, Cheng J, Li F, Chia D, Wei F, Wong DTW. Longitudinal Monitoring of EGFR and PIK3CA Mutations by Saliva-Based EFIRM in Advanced NSCLC Patients With Local Ablative Therapy and Osimertinib Treatment: Two Case Reports. Front Oncol. 2020; 10:1240. view on PubMed

Li F, Wei F, Huang WL, Lin CC, Li L, Shen MM, Yan Q, Liao W, Chia D, Tu M, Tang JH, Feng Z, Kim Y, Su WC, Wong DTW. Ultra-Short Circulating Tumor DNA (usctDNA) in Plasma and Saliva of Non-Small Cell Lung Cancer (NSCLC) Patients. Cancers (Basel). 2020 Jul 24; 12(8). view on PubMed

Tu M, Cheng J, Chen YL, Jea WC, Chen WL, Chen CJ, Ho CL, Huang WL, Lin CC, Su WC, Ye Q, Deignan J, Grody W, Li F, Chia D, Wei F, Liao W, Wong DTW, Strom CM. Electric Field-Induced Release and Measurement (EFIRM): Characterization and Technical Validation of a Novel Liquid Biopsy Platform in Plasma and Saliva. J Mol Diagn. 2020 08; 22(8):1050-1062. view on PubMed

Wang Z, Li F, Rufo J, Chen C, Yang S, Li L, Zhang J, Cheng J, Kim Y, Wu M, Abemayor E, Tu M, Chia D, Spruce R, Batis N, Mehanna H, Wong DTW, Huang TJ. Acoustofluidic Salivary Exosome Isolation: A Liquid Biopsy Compatible Approach for Human Papillomavirus-Associated Oropharyngeal Cancer Detection. J Mol Diagn. 2020 01; 22(1):50-59. view on PubMed

Kaczor-Urbanowicz KE, Wei F, Rao SL, Kim J, Shin H, Cheng J, Tu M, Wong DTW, Kim Y. Clinical validity of saliva and novel technology for cancer detection. Biochim Biophys Acta Rev Cancer. 2019 08; 1872(1):49-59. view on PubMed

Murillo OD, Thistlethwaite W, Rozowsky J, Subramanian SL, Lucero R, Shah N, Jackson AR, Srinivasan S, Chung A, Laurent CD, Kitchen RR, Galeev T, Warrell J, Diao JA, Welsh JA, Hanspers K, Riutta A, Burgstaller-Muehlbacher S, Shah RV, Yeri A, Jenkins LM, Ahsen ME, Cordon-Cardo C, Dogra N, Gifford SM, Smith JT, Stolovitzky G, Tewari AK, Wunsch BH, Yadav KK, Danielson KM, Filant J, Moeller C, Nejad P, Paul A, Simonson B, Wong DK, Zhang X, Balaj L, Gandhi R, Sood AK, Alexander RP, Wang L, Wu C, Wong DTW, Galas DJ, Van Keuren-Jensen K, Patel T, Jones JC, Das S, Cheung KH, Pico AR, Su AI, Raffai RL, Laurent LC, Roth ME, Gerstein MB, Milosavljevic A. exRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types and Their Carriers Present across Human Biofluids. Cell. 2019 04 04; 177(2):463-477.e15. view on PubMed

Cheng J, Nonaka T, Wong DTW. Salivary Exosomes as Nanocarriers for Cancer Biomarker Delivery. Materials (Basel). 2019 Feb 21; 12(4). view on PubMed

Ishikawa S, Wong DTW, Sugimoto M, Gleber-Netto FO, Li F, Tu M, Zhang Y, Akin D, Iino M. Identification of salivary metabolites for oral squamous cell carcinoma and oral epithelial dysplasia screening from persistent suspicious oral mucosal lesions. Clin Oral Investig. 2019 Sep; 23(9):3557-3563. view on PubMed

Wei F, Strom CM, Cheng J, Lin CC, Hsu CY, Soo Hoo GW, Chia D, Kim Y, Li F, Elashoff D, Grognan T, Tu M, Liao W, Xian R, Grody WW, Su WC, Wong DTW. Electric Field-Induced Release and Measurement Liquid Biopsy for Noninvasive Early Lung Cancer Assessment. J Mol Diagn. 2018 11; 20(6):738-742. view on PubMed

Li F, Yoshizawa JM, Kim KM, Kanjanapangka J, Grogan TR, Wang X, Elashoff DE, Ishikawa S, Chia D, Liao W, Akin D, Yan X, Lee MS, Choi R, Kim SM, Kang SY, Bae JM, Sohn TS, Lee JH, Choi MG, Min BH, Lee JH, Kim JJ, Kim Y, Kim S, Wong DTW. Discovery and Validation of Salivary Extracellular RNA Biomarkers for Noninvasive Detection of Gastric Cancer. Clin Chem. 2018 10; 64(10):1513-1521. view on PubMed

Kaczor-Urbanowicz KE, Trivedi HM, Lima PO, Camargo PM, Giannobile WV, Grogan TR, Gleber-Netto FO, Whiteman Y, Li F, Lee HJ, Dharia K, Aro K, Martin Carreras-Presas C, Amuthan S, Vartak M, Akin D, Al-Adbullah H, Bembey K, Klokkevold PR, Elashoff D, Barnes VM, Richter R, DeVizio W, Masters JG, Wong DTW. Salivary exRNA biomarkers to detect gingivitis and monitor disease regression. J Clin Periodontol. 2018 07; 45(7):806-817. view on PubMed

Li F, Kaczor-Urbanowicz KE, Sun J, Majem B, Lo HC, Kim Y, Koyano K, Rao SL, Kang SY, Kim SM, Kim KM, Kim S, Chia D, Elashoff D, Grogan TR, Xiao X, Wong DTW. Characterization of Human Salivary Extracellular RNA by Next-generation Sequencing. Clin Chem. 2018 07; 64(7):1085-1095. view on PubMed

Kaczor-Urbanowicz KE, Kim Y, Li F, Galeev T, Kitchen RR, Gerstein M, Koyano K, Jeong SH, Wang X, Elashoff D, Kang SY, Kim SM, Kim K, Kim S, Chia D, Xiao X, Rozowsky J, Wong DTW. Novel approaches for bioinformatic analysis of salivary RNA sequencing data for development. Bioinformatics. 2018 01 01; 34(1):1-8. view on PubMed

Nonaka T, Wong DTW. Saliva-Exosomics in Cancer: Molecular Characterization of Cancer-Derived Exosomes in Saliva. Enzymes. 2017; 42:125-151. view on PubMed

Zhang Y, Sun J, Li F, Grogan TR, Vergara JL, Luan Q, Park MS, Chia D, Elashoff D, Joshipura KJ, Wong DTW. Salivary extracellular RNA biomarkers for insulin resistance detection in hispanics. Diabetes Res Clin Pract. 2017 Oct; 132:85-94. view on PubMed

Majem B, Li F, Sun J, Wong DT. RNA Sequencing Analysis of Salivary Extracellular RNA. Methods Mol Biol. 2017; 1537:17-36. view on PubMed

Wang X, Kaczor-Urbanowicz KE, Wong DT. Salivary biomarkers in cancer detection. Med Oncol. 2017 Jan; 34(1):7. view on PubMed

Katsiougiannis S, Chia D, Kim Y, Singh RP, Wong DT. Saliva exosomes from pancreatic tumor-bearing mice modulate NK cell phenotype and antitumor cytotoxicity. FASEB J. 2017 03; 31(3):998-1010. view on PubMed

Kaczor-Urbanowicz KE, Martín Carreras-Presas C, Kaczor T, Tu M, Wei F, Garcia-Godoy F, Wong DT. Emerging technologies for salivaomics in cancer detection. J Cell Mol Med. 2017 04; 21(4):640-647. view on PubMed

Katsiougiannis S, Wong DT. The Proteomics of Saliva in Sjögren's Syndrome. Rheum Dis Clin North Am. 2016 08; 42(3):449-56. view on PubMed

Pu D, Liang H, Wei F, Akin D, Feng Z, Yan Q, Li Y, Zhen Y, Xu L, Dong G, Wan H, Dong J, Qiu X, Qin C, Zhu D, Wang X, Sun T, Zhang W, Li C, Tang X, Qiao Y, Wong DT, Zhou Q. Evaluation of a novel saliva-based epidermal growth factor receptor mutation detection for lung cancer: A pilot study. Thorac Cancer. 2016 Jul; 7(4):428-36. view on PubMed

Xiao H, Zhang Y, Kim Y, Kim S, Kim JJ, Kim KM, Yoshizawa J, Fan LY, Cao CX, Wong DT. Differential Proteomic Analysis of Human Saliva using Tandem Mass Tags Quantification for Gastric Cancer Detection. Sci Rep. 2016 Feb 25; 6:22165. view on PubMed

Gleber-Netto FO, Yakob M, Li F, Feng Z, Dai J, Kao HK, Chang YL, Chang KP, Wong DT. Salivary Biomarkers for Detection of Oral Squamous Cell Carcinoma in a Taiwanese Population. Clin Cancer Res. 2016 07 01; 22(13):3340-7. view on PubMed

Matse JH, Yoshizawa J, Wang X, Elashoff D, Bolscher JG, Veerman EC, Leemans CR, Pegtel DM, Pegtel MD, Wong DT, Bloemena E. Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. PLoS One. 2015; 10(11):e0142264. view on PubMed

Lin CC, Huang WL, Wei F, Su WC, Wong DT. Emerging platforms using liquid biopsy to detect EGFR mutations in lung cancer. Expert Rev Mol Diagn. 2015; 15(11):1427-40. view on PubMed

Huang WL, Wei F, Wong DT, Lin CC, Su WC. The Emergent Landscape of Detecting EGFR Mutations Using Circulating Tumor DNA in Lung Cancer. Biomed Res Int. 2015; 2015:340732. view on PubMed

Chai YD, Zhang L, Yang Y, Su T, Charugundla P, Ai J, Messadi D, Wong DT, Hu S. Discovery of potential serum protein biomarkers for lymph node metastasis in oral cancer. Head Neck. 2016 Jan; 38(1):118-25. view on PubMed

Lin X, Lo HC, Wong DT, Xiao X. Noncoding RNAs in human saliva as potential disease biomarkers. Front Genet. 2015; 6:175. view on PubMed

Majem B, Rigau M, Reventós J, Wong DT. Non-coding RNAs in saliva: emerging biomarkers for molecular diagnostics. Int J Mol Sci. 2015 Apr 17; 16(4):8676-98. view on PubMed

Wong DT. Salivary extracellular noncoding RNA: emerging biomarkers for molecular diagnostics. Clin Ther. 2015 Mar 01; 37(3):540-51. view on PubMed

Chiang SH, Thomas GA, Liao W, Grogan T, Buck RL, Fuentes L, Yakob M, Laughlin MJ, Schafer C, Nazmul-Hossain A, Wei F, Elashoff D, Slowey PD, Wong DT. RNAPro•SAL: a device for rapid and standardized collection of saliva RNA and proteins. Biotechniques. 2015 Feb; 58(2):69-76. view on PubMed

Wei F, Lin CC, Joon A, Feng Z, Troche G, Lira ME, Chia D, Mao M, Ho CL, Su WC, Wong DT. Noninvasive saliva-based EGFR gene mutation detection in patients with lung cancer. Am J Respir Crit Care Med. 2014 Nov 15; 190(10):1117-26. view on PubMed

Bahn JH, Zhang Q, Li F, Chan TM, Lin X, Kim Y, Wong DT, Xiao X. The landscape of microRNA, Piwi-interacting RNA, and circular RNA in human saliva. Clin Chem. 2015 Jan; 61(1):221-30. view on PubMed

Deutsch O, Krief G, Konttinen YT, Zaks B, Wong DT, Aframian DJ, Palmon A. Identification of Sjögren's syndrome oral fluid biomarker candidates following high-abundance protein depletion. Rheumatology (Oxford). 2015 May; 54(5):884-90. view on PubMed

Singh RP, Hasan S, Sharma S, Nagra S, Yamaguchi DT, Wong DT, Hahn BH, Hossain A. Th17 cells in inflammation and autoimmunity. Autoimmun Rev. 2014 Dec; 13(12):1174-81. view on PubMed

Yakob M, Fuentes L, Wang MB, Abemayor E, Wong DT. Salivary biomarkers for detection of oral squamous cell carcinoma - current state and recent advances. Curr Oral Health Rep. 2014 Jun 01; 1(2):133-141. view on PubMed

Zhang Y, Sun J, Lin CC, Abemayor E, Wang MB, Wong DT. The emerging landscape of salivary diagnostics. Oral Health Dent Manag. 2014 Jun; 13(2):200-10. view on PubMed

Schafer CA, Schafer JJ, Yakob M, Lima P, Camargo P, Wong DT. Saliva diagnostics: utilizing oral fluids to determine health status. Monogr Oral Sci. 2014; 24:88-98. view on PubMed

Yoshizawa JM, Schafer CA, Schafer JJ, Farrell JJ, Paster BJ, Wong DT. Salivary biomarkers: toward future clinical and diagnostic utilities. Clin Microbiol Rev. 2013 Oct; 26(4):781-91. view on PubMed

Lau C, Kim Y, Chia D, Spielmann N, Eibl G, Elashoff D, Wei F, Lin YL, Moro A, Grogan T, Chiang S, Feinstein E, Schafer C, Farrell J, Wong DT. Role of pancreatic cancer-derived exosomes in salivary biomarker development. J Biol Chem. 2013 Sep 13; 288(37):26888-97. view on PubMed

Wei F, Yang J, Wong DT. Detection of exosomal biomarker by electric field-induced release and measurement (EFIRM). Biosens Bioelectron. 2013 Jun 15; 44:115-21. view on PubMed

Yoshizawa JM, Wong DT. Salivary microRNAs and oral cancer detection. Methods Mol Biol. 2013; 936:313-24. view on PubMed

Bonne NJ, Wong DT. Salivary biomarker development using genomic, proteomic and metabolomic approaches. Genome Med. 2012; 4(10):82. view on PubMed

Kim JJ, Khalid O, Vo S, Sun HH, Wong DT, Kim Y. A novel regulatory factor recruits the nucleosome remodeling complex to wingless integrated (Wnt) signaling gene promoters in mouse embryonic stem cells. J Biol Chem. 2012 Nov 30; 287(49):41103-17. view on PubMed

Wong DT. Salivaomics. J Am Dent Assoc. 2012 Oct; 143(10 Suppl):19S-24S. view on PubMed

Spielmann N, Ilsley D, Gu J, Lea K, Brockman J, Heater S, Setterquist R, Wong DT. The human salivary RNA transcriptome revealed by massively parallel sequencing. Clin Chem. 2012 Sep; 58(9):1314-21. view on PubMed

Deshpande AM, Khalid O, Kim JJ, Kim Y, Lindgren A, Clark AT, Wong DT. Cdk2ap2 is a novel regulator for self-renewal of murine embryonic stem cells. Stem Cells Dev. 2012 Nov 01; 21(16):3010-8. view on PubMed

Zhang L, Xiao H, Zhou H, Santiago S, Lee JM, Garon EB, Yang J, Brinkmann O, Yan X, Akin D, Chia D, Elashoff D, Park NH, Wong DTW. Development of transcriptomic biomarker signature in human saliva to detect lung cancer. Cell Mol Life Sci. 2012 Oct; 69(19):3341-3350. view on PubMed

Elashoff D, Zhou H, Reiss J, Wang J, Xiao H, Henson B, Hu S, Arellano M, Sinha U, Le A, Messadi D, Wang M, Nabili V, Lingen M, Morris D, Randolph T, Feng Z, Akin D, Kastratovic DA, Chia D, Abemayor E, Wong DT. Prevalidation of salivary biomarkers for oral cancer detection. Cancer Epidemiol Biomarkers Prev. 2012 Apr; 21(4):664-72. view on PubMed

Farrell JJ, Zhang L, Zhou H, Chia D, Elashoff D, Akin D, Paster BJ, Joshipura K, Wong DT. Variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. Gut. 2012 Apr; 61(4):582-8. view on PubMed

Lee YH, Zhou H, Reiss JK, Yan X, Zhang L, Chia D, Wong DT. Direct saliva transcriptome analysis. Clin Chem. 2011 Sep; 57(9):1295-302. view on PubMed

Hu S, Vissink A, Arellano M, Roozendaal C, Zhou H, Kallenberg CG, Wong DT. Identification of autoantibody biomarkers for primary Sjögren's syndrome using protein microarrays. Proteomics. 2011 Apr; 11(8):1499-507. view on PubMed

Brinkmann O, Zhang L, Giannobile WV, Wong DT. Salivary biomarkers for periodontal disease diagnostics. Expert Opin Med Diagn. 2011 Jan; 5(1):25-35. view on PubMed

Zhang L, Xiao H, Karlan S, Zhou H, Gross J, Elashoff D, Akin D, Yan X, Chia D, Karlan B, Wong DT. Discovery and preclinical validation of salivary transcriptomic and proteomic biomarkers for the non-invasive detection of breast cancer. PLoS One. 2010 Dec 31; 5(12):e15573. view on PubMed

Brinkmann O, Kastratovic DA, Dimitrijevic MV, Konstantinovic VS, Jelovac DB, Antic J, Nesic VS, Markovic SZ, Martinovic ZR, Akin D, Spielmann N, Zhou H, Wong DT. Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population. Oral Oncol. 2011 Jan; 47(1):51-5. view on PubMed

Ai J, Smith B, Wong DT. Saliva Ontology: an ontology-based framework for a Salivaomics Knowledge Base. BMC Bioinformatics. 2010 Jun 03; 11:302. view on PubMed

Ryan CM, Souda P, Halgand F, Wong DT, Loo JA, Faull KF, Whitelegge JP. Confident assignment of intact mass tags to human salivary cystatins using top-down Fourier-transform ion cyclotron resonance mass spectrometry. J Am Soc Mass Spectrom. 2010 Jun; 21(6):908-17. view on PubMed

Halgand F, Zabrouskov V, Bassilian S, Souda P, Wong DT, Loo JA, Faull KF, Whitelegge JP. Micro-heterogeneity of human saliva Peptide P-C characterized by high-resolution top-down Fourier-transform mass spectrometry. J Am Soc Mass Spectrom. 2010 May; 21(5):868-77. view on PubMed

Zhang L, Farrell JJ, Zhou H, Elashoff D, Akin D, Park NH, Chia D, Wong DT. Salivary transcriptomic biomarkers for detection of resectable pancreatic cancer. Gastroenterology. 2010 Mar; 138(3):949-57.e1-7. view on PubMed

Wei F, Liao W, Xu Z, Yang Y, Wong DT, Ho CM. Bio/abiotic interface constructed from nanoscale DNA dendrimer and conducting polymer for ultrasensitive biomolecular diagnosis. Small. 2009 Aug 03; 5(15):1784-90. view on PubMed

Lee YH, Wong DT. Saliva: an emerging biofluid for early detection of diseases. Am J Dent. 2009 Aug; 22(4):241-8. view on PubMed

Kim Y, Deshpande A, Dai Y, Kim JJ, Lindgren A, Conway A, Clark AT, Wong DT. Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. J Biol Chem. 2009 Aug 28; 284(35):23405-14. view on PubMed

Gao K, Zhou H, Zhang L, Lee JW, Zhou Q, Hu S, Wolinsky LE, Farrell J, Eibl G, Wong DT. Systemic disease-induced salivary biomarker profiles in mouse models of melanoma and non-small cell lung cancer. PLoS One. 2009 Jun 11; 4(6):e5875. view on PubMed

Kim Y, McBride J, Kimlin L, Pae EK, Deshpande A, Wong DT. Targeted inactivation of p12, CDK2 associating protein 1, leads to early embryonic lethality. PLoS One. 2009; 4(2):e4518. view on PubMed

Hu S, Wong DT. Lectin microarray. Proteomics Clin Appl. 2009 Feb; 3(2):148-54. view on PubMed

Hu S, Zhou M, Jiang J, Wang J, Elashoff D, Gorr S, Michie SA, Spijkervet FK, Bootsma H, Kallenberg CG, Vissink A, Horvath S, Wong DT. Systems biology analysis of Sjögren's syndrome and mucosa-associated lymphoid tissue lymphoma in parotid glands. Arthritis Rheum. 2009 Jan; 60(1):81-92. view on PubMed

Deshpande AM, Dai YS, Kim Y, Kim J, Kimlin L, Gao K, Wong DT. Cdk2ap1 is required for epigenetic silencing of Oct4 during murine embryonic stem cell differentiation. J Biol Chem. 2009 Mar 06; 284(10):6043-7. view on PubMed

Jiang J, Park NJ, Hu S, Wong DT. A universal pre-analytic solution for concurrent stabilization of salivary proteins, RNA and DNA at ambient temperature. Arch Oral Biol. 2009 Mar; 54(3):268-73. view on PubMed

Hu S, Arellano M, Boontheung P, Wang J, Zhou H, Jiang J, Elashoff D, Wei R, Loo JA, Wong DT. Salivary proteomics for oral cancer biomarker discovery. Clin Cancer Res. 2008 Oct 01; 14(19):6246-52. view on PubMed

Brooks MN, Wang J, Li Y, Zhang R, Elashoff D, Wong DT. Salivary protein factors are elevated in breast cancer patients. Mol Med Rep. 2008 May-Jun; 1(3):375-8. view on PubMed

Hu Z, Zimmermann BG, Zhou H, Wang J, Henson BS, Yu W, Elashoff D, Krupp G, Wong DT. Exon-level expression profiling: a comprehensive transcriptome analysis of oral fluids. Clin Chem. 2008 May; 54(5):824-32. view on PubMed

Wong DT. Salivary Diagnostics: Amazing as it might seem, doctors can detect and monitor diseases using molecules found in a sample of spit. Am Sci. 2008 Jan 01; 96(1):37-43. view on PubMed

Zimmermann BG, Wong DT. Salivary mRNA targets for cancer diagnostics. Oral Oncol. 2008 May; 44(5):425-9. view on PubMed

Whitelegge JP, Zabrouskov V, Halgand F, Souda P, Bassilian S, Yan W, Wolinsky L, Loo JA, Wong DT, Faull KF. Protein-Sequence Polymorphisms and Post-translational Modifications in Proteins from Human Saliva using Top-Down Fourier-transform Ion Cyclotron Resonance Mass Spectrometry. Int J Mass Spectrom. 2007 Dec 01; 268(2-3):190-197. view on PubMed

Hu S, Yen Y, Ann D, Wong DT. Implications of salivary proteomics in drug discovery and development: a focus on cancer drug discovery. Drug Discov Today. 2007 Nov; 12(21-22):911-6. view on PubMed

Hu S, Wong DT. Oral cancer proteomics. Curr Opin Mol Ther. 2007 Oct; 9(5):467-76. view on PubMed

Zimmermann BG, Park NJ, Wong DT. Genomic targets in saliva. Ann N Y Acad Sci. 2007 Mar; 1098:184-91. view on PubMed

Hu S, Loo JA, Wong DT. Human saliva proteome analysis. Ann N Y Acad Sci. 2007 Mar; 1098:323-9. view on PubMed

Peng H, Shintani S, Kim Y, Wong DT. Loss of p12CDK2-AP1 expression in human oral squamous cell carcinoma with disrupted transforming growth factor-beta-Smad signaling pathway. Neoplasia. 2006 Dec; 8(12):1028-36. view on PubMed

Zhou X, Temam S, Oh M, Pungpravat N, Huang BL, Mao L, Wong DT. Global expression-based classification of lymph node metastasis and extracapsular spread of oral tongue squamous cell carcinoma. Neoplasia. 2006 Nov; 8(11):925-32. view on PubMed

Park NJ, Li Y, Yu T, Brinkman BM, Wong DT. Characterization of RNA in saliva. Clin Chem. 2006 Jun; 52(6):988-94. view on PubMed

Wong DT. Salivary diagnostics powered by nanotechnologies, proteomics and genomics. J Am Dent Assoc. 2006 Mar; 137(3):313-21. view on PubMed

Li Y, Elashoff D, Oh M, Sinha U, St John MA, Zhou X, Abemayor E, Wong DT. Serum circulating human mRNA profiling and its utility for oral cancer detection. J Clin Oncol. 2006 Apr 10; 24(11):1754-60. view on PubMed

Zhou X, Yu T, Cole SW, Wong DT. Advancement in characterization of genomic alterations for improved diagnosis, treatment and prognostics in cancer. Expert Rev Mol Diagn. 2006 Jan; 6(1):39-50. view on PubMed

Gu F, Lux R, Du-Thumm L, Stokes I, Kreth J, Anderson MH, Wong DT, Wolinsky L, Sullivan R, Shi W. In situ and non-invasive detection of specific bacterial species in oral biofilms using fluorescently labeled monoclonal antibodies. J Microbiol Methods. 2005 Aug; 62(2):145-60. view on PubMed

Figueiredo ML, Kim Y, St John MA, Wong DT. p12CDK2-AP1 gene therapy strategy inhibits tumor growth in an in vivo mouse model of head and neck cancer. Clin Cancer Res. 2005 May 15; 11(10):3939-48. view on PubMed

Zhou X, Rao NP, Cole SW, Mok SC, Chen Z, Wong DT. Progress in concurrent analysis of loss of heterozygosity and comparative genomic hybridization utilizing high density single nucleotide polymorphism arrays. Cancer Genet Cytogenet. 2005 May; 159(1):53-7. view on PubMed

Kim Y, Ohyama H, Patel V, Figueiredo M, Wong DT. Mutation of Cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect. J Biol Chem. 2005 Jun 17; 280(24):23273-9. view on PubMed

Kim Y, McBride J, Zhang R, Zhou X, Wong DT. p12(CDK2-AP1) mediates DNA damage responses induced by cisplatin. Oncogene. 2005 Jan 13; 24(3):407-18. view on PubMed

Kim Y, Shintani S, Kohno Y, Zhang R, Wong DT. Cyclin G2 dysregulation in human oral cancer. Cancer Res. 2004 Dec 15; 64(24):8980-6. view on PubMed

Li Y, St John MA, Zhou X, Kim Y, Sinha U, Jordan RC, Eisele D, Abemayor E, Elashoff D, Park NH, Wong DT. Salivary transcriptome diagnostics for oral cancer detection. Clin Cancer Res. 2004 Dec 15; 10(24):8442-50. view on PubMed

Hu S, Denny P, Denny P, Xie Y, Loo JA, Wolinsky LE, Li Y, McBride J, Ogorzalek Loo RR, Navazesh M, Wong DT. Differentially expressed protein markers in human submandibular and sublingual secretions. Int J Oncol. 2004 Nov; 25(5):1423-30. view on PubMed

Zhou X, Mok SC, Chen Z, Li Y, Wong DT. Concurrent analysis of loss of heterozygosity (LOH) and copy number abnormality (CNA) for oral premalignancy progression using the Affymetrix 10K SNP mapping array. Hum Genet. 2004 Sep; 115(4):327-30. view on PubMed

St John MA, Li Y, Zhou X, Denny P, Ho CM, Montemagno C, Shi W, Qi F, Wu B, Sinha U, Jordan R, Wolinsky L, Park NH, Liu H, Abemayor E, Wong DT. Interleukin 6 and interleukin 8 as potential biomarkers for oral cavity and oropharyngeal squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 2004 Aug; 130(8):929-35. view on PubMed

Buajeeb W, Zhang X, Ohyama H, Han D, Surarit R, Kim Y, Wong DT. Interaction of the CDK2-associated protein-1, p12(DOC-1/CDK2AP1), with its homolog, p14(DOC-1R). Biochem Biophys Res Commun. 2004 Mar 19; 315(4):998-1003. view on PubMed

Zhou X, Cole SW, Hu S, Wong DT. Detection of DNA copy number abnormality by microarray expression analysis. Hum Genet. 2004 Apr; 114(5):464-7. view on PubMed

Hu MG, Hu GF, Kim Y, Tsuji T, McBride J, Hinds P, Wong DT. Role of p12(CDK2-AP1) in transforming growth factor-beta1-mediated growth suppression. Cancer Res. 2004 Jan 15; 64(2):490-9. view on PubMed

Kim Y, Donoff RB, Wong DT, Todd R. The nucleotide: DNA sequencing and its clinical application. J Oral Maxillofac Surg. 2002 Aug; 60(8):924-30. view on PubMed

Kim Y, Flynn TR, Donoff RB, Wong DT, Todd R. The gene: the polymerase chain reaction and its clinical application. J Oral Maxillofac Surg. 2002 Jul; 60(7):808-15. view on PubMed