Robert Clubb*
Research Summary: Bacterial pathogens mount successful infections by displaying virulence factors that enable them to adhere to host tissues, evade the immune response, invade host cells and acquire essential nutrients. I am interested in understanding the molecular mechanisms that underpin virulence factor display and function, and I hope to translate my laboratory’s research findings into practical applications.
Websites:
Publications:
Mahoney BJ, Lyman LR, Ford J, Soule J, Cheung NA, Goring AK, Ellis-Guardiola K, Collazo MJ, Cascio D, Ton-That H, Schmitt MP, Clubb RT. Molecular basis of hemoglobin binding and heme removal in Corynebacterium diphtheriae. Proc Natl Acad Sci U S A. 2025 Jan 07; 122(1):e2411833122. view on PubMed
Goring AK, Hale S, Dasika P, Chen Y, Clubb RT, Loo JA. The Exoproteome and Surfaceome of Toxigenic Corynebacterium diphtheriae 1737 and Its Response to Iron Restriction and Growth on Human Hemoglobin. J Proteome Res. 2025 Jan 03; 24(1):77-93. view on PubMed
Minor CM, Takayesu A, Ha SM, Salwinski L, Sawaya MR, Pellegrini M, Clubb RT. A genomic analysis reveals the diversity of cellulosome displaying bacteria. Front Microbiol. 2024; 15:1473396. view on PubMed
Takayesu A, Mahoney BJ, Goring AK, Jessup T, Ogorzalek Loo RR, Loo JA, Clubb RT. Insight into the autoproteolysis mechanism of the RsgI9 anti-σ factor from Clostridium thermocellum. Proteins. 2024 Aug; 92(8):946-958. view on PubMed
Cheung NA, Song M, Sue CK, Clubb RT. Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography. Methods Mol Biol. 2024; 2727:135-143. view on PubMed
Mahoney BJ, Goring AK, Wang Y, Dasika P, Zhou A, Grossbard E, Cascio D, Loo JA, Clubb RT. Development and atomic structure of a new fluorescence-based sensor to probe heme transfer in bacterial pathogens. J Inorg Biochem. 2023 12; 249:112368. view on PubMed
Sue CK, Cheung NA, Mahoney BJ, McConnell SA, Scully JM, Fu JY, Chang C, Ton-That H, Loo JA, Clubb RT. The basal and major pilins in the Corynebacterium diphtheriae SpaA pilus adopt similar structures that competitively react with the pilin polymerase. Biopolymers. 2024 Jan; 115(1):e23539. view on PubMed
Sue CK, Cheung NA, Mahoney BJ, McConnell SA, Scully JM, Fu JY, Chang C, Ton-That H, Loo JA, Clubb RT. The Basal and Major Pilins in the Corynebacterium diphtheriae SpaA Pilus Adopt Similar Structures that Competitively React with the Pilin Polymerase. bioRxiv. 2023 Feb 23. view on PubMed
Macdonald R, Mahoney BJ, Soule J, Goring AK, Ford J, Loo JA, Cascio D, Clubb RT. The Shr receptor from Streptococcus pyogenes uses a cap and release mechanism to acquire heme-iron from human hemoglobin. Proc Natl Acad Sci U S A. 2023 01 31; 120(5):e2211939120. view on PubMed
Clayton J, Ellis-Guardiola K, Mahoney BJ, Soule J, Liu W, Clubb RT, Wereszczynski J. Directed Inter-domain Motions Enable the IsdH Staphylococcus aureus Receptor to Rapidly Extract Heme from Human Hemoglobin. J Mol Biol. 2022 06 30; 434(12):167623. view on PubMed
Mahoney BJ, Takayesu A, Zhou A, Cascio D, Clubb RT. The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing. Proteins. 2022 07; 90(7):1457-1467. view on PubMed
Martinez OE, Mahoney BJ, Goring AK, Yi SW, Tran DP, Cascio D, Phillips ML, Muthana MM, Chen X, Jung ME, Loo JA, Clubb RT. Insight into the molecular basis of substrate recognition by the wall teichoic acid glycosyltransferase TagA. J Biol Chem. 2022 02; 298(2):101464. view on PubMed
Ellis-Guardiola K, Soule J, Clubb RT. Methods for the Extraction of Heme Prosthetic Groups from Hemoproteins. Bio Protoc. 2021 09 20; 11(18):e4156. view on PubMed
McConnell SA, McAllister RA, Amer BR, Mahoney BJ, Sue CK, Chang C, Ton-That H, Clubb RT. Sortase-assembled pili in Corynebacterium diphtheriae are built using a latch mechanism. Proc Natl Acad Sci U S A. 2021 03 23; 118(12). view on PubMed
Ellis-Guardiola K, Mahoney BJ, Clubb RT. NEAr Transporter (NEAT) Domains: Unique Surface Displayed Heme Chaperones That Enable Gram-Positive Bacteria to Capture Heme-Iron From Hemoglobin. Front Microbiol. 2020; 11:607679. view on PubMed
Sue CK, McConnell SA, Ellis-Guardiola K, Muroski JM, McAllister RA, Yu J, Alvarez AI, Chang C, Ogorzalek Loo RR, Loo JA, Ton-That H, Clubb RT. Kinetics and Optimization of the Lysine-Isopeptide Bond Forming Sortase Enzyme from Corynebacterium diphtheriae. Bioconjug Chem. 2020 06 17; 31(6):1624-1634. view on PubMed
Gosschalk JE, Chang C, Sue CK, Siegel SD, Wu C, Kattke MD, Yi SW, Damoiseaux R, Jung ME, Ton-That H, Clubb RT. A Cell-based Screen in Actinomyces oris to Identify Sortase Inhibitors. Sci Rep. 2020 05 22; 10(1):8520. view on PubMed
McConnell SA, Cannon KA, Morgan C, McAllister R, Amer BR, Clubb RT, Yeates TO. Designed Protein Cages as Scaffolds for Building Multienzyme Materials. ACS Synth Biol. 2020 02 21; 9(2):381-391. view on PubMed
Ellis-Guardiola K, Clayton J, Pham C, Mahoney BJ, Wereszczynski J, Clubb RT. The Staphylococcus aureus IsdH Receptor Forms a Dynamic Complex with Human Hemoglobin that Triggers Heme Release via Two Distinct Hot Spots. J Mol Biol. 2020 02 14; 432(4):1064-1082. view on PubMed
Chang C, Wu C, Osipiuk J, Siegel SD, Zhu S, Liu X, Joachimiak A, Clubb RT, Das A, Ton-That H. Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes. Proc Natl Acad Sci U S A. 2019 09 03; 116(36):18041-18049. view on PubMed
Macdonald R, Mahoney BJ, Ellis-Guardiola K, Maresso A, Clubb RT. NMR experiments redefine the hemoglobin binding properties of bacterial NEAr-iron Transporter domains. Protein Sci. 2019 08; 28(8):1513-1523. view on PubMed
Tsui HS, Pham NVB, Amer BR, Bradley MC, Gosschalk JE, Gallagher-Jones M, Ibarra H, Clubb RT, Blaby-Haas CE, Clarke CF. Human COQ10A and COQ10B are distinct lipid-binding START domain proteins required for coenzyme Q function. J Lipid Res. 2019 07; 60(7):1293-1310. view on PubMed
Kattke MD, Gosschalk JE, Martinez OE, Kumar G, Gale RT, Cascio D, Sawaya MR, Philips M, Brown ED, Clubb RT. Structure and mechanism of TagA, a novel membrane-associated glycosyltransferase that produces wall teichoic acids in pathogenic bacteria. PLoS Pathog. 2019 04; 15(4):e1007723. view on PubMed
Siegel SD, Amer BR, Wu C, Sawaya MR, Gosschalk JE, Clubb RT, Ton-That H. Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein. mBio. 2019 02 19; 10(1). view on PubMed
Macdonald R, Cascio D, Collazo MJ, Phillips M, Clubb RT. The Streptococcus pyogenes Shr protein captures human hemoglobin using two structurally unique binding domains. J Biol Chem. 2018 11 23; 293(47):18365-18377. view on PubMed
McConnell SA, Amer BR, Muroski J, Fu J, Chang C, Ogorzalek Loo RR, Loo JA, Osipiuk J, Ton-That H, Clubb RT. Protein Labeling via a Specific Lysine-Isopeptide Bond Using the Pilin Polymerizing Sortase from Corynebacterium diphtheriae. J Am Chem Soc. 2018 07 11; 140(27):8420-8423. view on PubMed
Chang C, Amer BR, Osipiuk J, McConnell SA, Huang IH, Hsieh V, Fu J, Nguyen HH, Muroski J, Flores E, Ogorzalek Loo RR, Loo JA, Putkey JA, Joachimiak A, Das A, Clubb RT, Ton-That H. In vitro reconstitution of sortase-catalyzed pilus polymerization reveals structural elements involved in pilin cross-linking. Proc Natl Acad Sci U S A. 2018 06 12; 115(24):E5477-E5486. view on PubMed
Huang GL, Gosschalk JE, Kim YS, Ogorzalek Loo RR, Clubb RT. Stabilizing displayed proteins on vegetative Bacillus subtilis cells. Appl Microbiol Biotechnol. 2018 Aug; 102(15):6547-6565. view on PubMed
Sjodt M, Macdonald R, Marshall JD, Clayton J, Olson JS, Phillips M, Gell DA, Wereszczynski J, Clubb RT. Energetics underlying hemin extraction from human hemoglobin by Staphylococcus aureus. J Biol Chem. 2018 05 04; 293(18):6942-6957. view on PubMed
Jacobitz AW, Kattke MD, Wereszczynski J, Clubb RT. Sortase Transpeptidases: Structural Biology and Catalytic Mechanism. Adv Protein Chem Struct Biol. 2017; 109:223-264. view on PubMed
Sjodt M, Clubb RT. Nitroxide Labeling of Proteins and the Determination of Paramagnetic Relaxation Derived Distance Restraints for NMR Studies. Bio Protoc. 2017 Apr 05; 7(7). view on PubMed
Chan AH, Yi SW, Weiner EM, Amer BR, Sue CK, Wereszczynski J, Dillen CA, Senese S, Torres JZ, McCammon JA, Miller LS, Jung ME, Clubb RT. NMR structure-based optimization of Staphylococcus aureus sortase A pyridazinone inhibitors. Chem Biol Drug Des. 2017 Sep; 90(3):327-344. view on PubMed
Kattke MD, Chan AH, Duong A, Sexton DL, Sawaya MR, Cascio D, Elliot MA, Clubb RT. Crystal Structure of the Streptomyces coelicolor Sortase E1 Transpeptidase Provides Insight into the Binding Mode of the Novel Class E Sorting Signal. PLoS One. 2016; 11(12):e0167763. view on PubMed
Jacobitz AW, Naziga EB, Yi SW, McConnell SA, Peterson R, Jung ME, Clubb RT, Wereszczynski J. The "Lid" in the Streptococcus pneumoniae SrtC1 Sortase Adopts a Rigid Structure that Regulates Substrate Access to the Active Site. J Phys Chem B. 2016 08 25; 120(33):8302-12. view on PubMed
Amer BR, Macdonald R, Jacobitz AW, Liauw B, Clubb RT. Rapid addition of unlabeled silent solubility tags to proteins using a new substrate-fused sortase reagent. J Biomol NMR. 2016 Mar; 64(3):197-205. view on PubMed
Tekeste SS, Wilkinson TA, Weiner EM, Xu X, Miller JT, Le Grice SF, Clubb RT, Chow SA. Interaction between Reverse Transcriptase and Integrase Is Required for Reverse Transcription during HIV-1 Replication. J Virol. 2015 Dec; 89(23):12058-69. view on PubMed
Chan AH, Yi SW, Terwilliger AL, Maresso AW, Jung ME, Clubb RT. Structure of the Bacillus anthracis Sortase A Enzyme Bound to Its Sorting Signal: A FLEXIBLE AMINO-TERMINAL APPENDAGE MODULATES SUBSTRATE ACCESS. J Biol Chem. 2015 Oct 16; 290(42):25461-74. view on PubMed
Zilbermintz L, Leonardi W, Jeong SY, Sjodt M, McComb R, Ho CL, Retterer C, Gharaibeh D, Zamani R, Soloveva V, Bavari S, Levitin A, West J, Bradley KA, Clubb RT, Cohen SN, Gupta V, Martchenko M. Identification of agents effective against multiple toxins and viruses by host-oriented cell targeting. Sci Rep. 2015 Aug 27; 5:13476. view on PubMed
Macdonald R, Sarkar D, Amer BR, Clubb RT. Solution structure of the PhoP DNA-binding domain from Mycobacterium tuberculosis. J Biomol NMR. 2015 Sep; 63(1):111-7. view on PubMed
Dickson CF, Jacques DA, Clubb RT, Guss JM, Gell DA. The structure of haemoglobin bound to the haemoglobin receptor IsdH from Staphylococcus aureus shows disruption of the native α-globin haem pocket. Acta Crystallogr D Biol Crystallogr. 2015 Jun; 71(Pt 6):1295-306. view on PubMed
Sjodt M, Macdonald R, Spirig T, Chan AH, Dickson CF, Fabian M, Olson JS, Gell DA, Clubb RT. The PRE-Derived NMR Model of the 38.8-kDa Tri-Domain IsdH Protein from Staphylococcus aureus Suggests That It Adaptively Recognizes Human Hemoglobin. J Mol Biol. 2016 Mar 27; 428(6):1107-1129. view on PubMed
Zhang J, Reza Malmirchegini G, Clubb RT, Loo JA. Native top-down mass spectrometry for the structural characterization of human hemoglobin. Eur J Mass Spectrom (Chichester). 2015; 21(3):221-31. view on PubMed
Amer BR, Clubb RT. A sweet new role for LCP enzymes in protein glycosylation. Mol Microbiol. 2014 Dec; 94(6):1197-200. view on PubMed
Malmirchegini GR, Sjodt M, Shnitkind S, Sawaya MR, Rosinski J, Newton SM, Klebba PE, Clubb RT. Novel mechanism of hemin capture by Hbp2, the hemoglobin-binding hemophore from Listeria monocytogenes. J Biol Chem. 2014 Dec 12; 289(50):34886-99. view on PubMed
Wolahan SM, Li Z, Hsu CH, Huang SJ, Clubb R, Hwang LP, Lin YY. A robust approach to correct for pronounced errors in temperature measurements by controlling radiation damping feedback fields in solution NMR. J Magn Reson. 2014 Nov; 248:19-22. view on PubMed
Twu O, Dessí D, Vu A, Mercer F, Stevens GC, de Miguel N, Rappelli P, Cocco AR, Clubb RT, Fiori PL, Johnson PJ. Trichomonas vaginalis homolog of macrophage migration inhibitory factor induces prostate cell growth, invasiveness, and inflammatory responses. Proc Natl Acad Sci U S A. 2014 Jun 03; 111(22):8179-84. view on PubMed
Jacobitz AW, Wereszczynski J, Yi SW, Amer BR, Huang GL, Nguyen AV, Sawaya MR, Jung ME, McCammon JA, Clubb RT. Structural and computational studies of the Staphylococcus aureus sortase B-substrate complex reveal a substrate-stabilized oxyanion hole. J Biol Chem. 2014 Mar 28; 289(13):8891-902. view on PubMed
Dickson CF, Kumar KK, Jacques DA, Malmirchegini GR, Spirig T, Mackay JP, Clubb RT, Guss JM, Gell DA. Structure of the hemoglobin-IsdH complex reveals the molecular basis of iron capture by Staphylococcus aureus. J Biol Chem. 2014 Mar 07; 289(10):6728-6738. view on PubMed
Huang GL, Anderson TD, Clubb RT. Engineering microbial surfaces to degrade lignocellulosic biomass. Bioengineered. 2014 Mar-Apr; 5(2):96-106. view on PubMed
Chan AH, Wereszczynski J, Amer BR, Yi SW, Jung ME, McCammon JA, Clubb RT. Discovery of Staphylococcus aureus sortase A inhibitors using virtual screening and the relaxed complex scheme. Chem Biol Drug Des. 2013 Oct; 82(4):418-28. view on PubMed
Ran Y, Malmirchegini GR, Clubb RT, Lei B. Axial ligand replacement mechanism in heme transfer from streptococcal heme-binding protein Shp to HtsA of the HtsABC transporter. Biochemistry. 2013 Sep 17; 52(37):6537-47. view on PubMed
Kappel K, Wereszczynski J, Clubb RT, McCammon JA. The binding mechanism, multiple binding modes, and allosteric regulation of Staphylococcus aureus Sortase A probed by molecular dynamics simulations. Protein Sci. 2012 Dec; 21(12):1858-71. view on PubMed
Spirig T, Malmirchegini GR, Zhang J, Robson SA, Sjodt M, Liu M, Krishna Kumar K, Dickson CF, Gell DA, Lei B, Loo JA, Clubb RT. Staphylococcus aureus uses a novel multidomain receptor to break apart human hemoglobin and steal its heme. J Biol Chem. 2013 Jan 11; 288(2):1065-78. view on PubMed
Robson SA, Jacobitz AW, Phillips ML, Clubb RT. Solution structure of the sortase required for efficient production of infectious Bacillus anthracis spores. Biochemistry. 2012 Oct 09; 51(40):7953-63. view on PubMed
Sam MD, Clubb RT. Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies. Methods Mol Biol. 2012; 831:219-32. view on PubMed
Spirig T, Clubb RT. Backbone 1H, 13C and 15N resonance assignments of the 39 kDa staphylococcal hemoglobin receptor IsdH. Biomol NMR Assign. 2012 Oct; 6(2):169-72. view on PubMed
Spirig T, Weiner EM, Clubb RT. Sortase enzymes in Gram-positive bacteria. Mol Microbiol. 2011 Dec; 82(5):1044-59. view on PubMed
Krishna Kumar K, Jacques DA, Pishchany G, Caradoc-Davies T, Spirig T, Malmirchegini GR, Langley DB, Dickson CF, Mackay JP, Clubb RT, Skaar EP, Guss JM, Gell DA. Structural basis for hemoglobin capture by Staphylococcus aureus cell-surface protein, IsdH. J Biol Chem. 2011 Nov 04; 286(44):38439-38447. view on PubMed
Villareal VA, Spirig T, Robson SA, Liu M, Lei B, Clubb RT. Transient weak protein-protein complexes transfer heme across the cell wall of Staphylococcus aureus. J Am Chem Soc. 2011 Sep 14; 133(36):14176-9. view on PubMed
Januszyk K, Fleissner MR, Atchabahian L, Shieh FK, Altenbach C, Martin SL, Guo F, Hubbell WL, Clubb RT. Site-directed spin labeling electron paramagnetic resonance study of the ORF1 protein from a mouse L1 retrotransposon. Protein Sci. 2011 Jul; 20(7):1231-43. view on PubMed
Ramey JD, Villareal VA, Ng C, Ward SC, Xiong JP, Clubb RT, Bradley KA. Anthrax toxin receptor 1/tumor endothelial marker 8: mutation of conserved inserted domain residues overrides cytosolic control of protective antigen binding. Biochemistry. 2010 Aug 31; 49(34):7403-10. view on PubMed
Robson SA, Peterson R, Bouchard LS, Villareal VA, Clubb RT. A heteronuclear zero quantum coherence Nz-exchange experiment that resolves resonance overlap and its application to measure the rates of heme binding to the IsdC protein. J Am Chem Soc. 2010 Jul 21; 132(28):9522-3. view on PubMed
Senturia R, Faller M, Yin S, Loo JA, Cascio D, Sawaya MR, Hwang D, Clubb RT, Guo F. Structure of the dimerization domain of DiGeorge critical region 8. Protein Sci. 2010 Jul; 19(7):1354-65. view on PubMed
Weiner EM, Robson S, Marohn M, Clubb RT. The Sortase A enzyme that attaches proteins to the cell wall of Bacillus anthracis contains an unusual active site architecture. J Biol Chem. 2010 Jul 23; 285(30):23433-43. view on PubMed
Suree N, Yi SW, Thieu W, Marohn M, Damoiseaux R, Chan A, Jung ME, Clubb RT. Discovery and structure-activity relationship analysis of Staphylococcus aureus sortase A inhibitors. Bioorg Med Chem. 2009 Oct 15; 17(20):7174-85. view on PubMed
Suree N, Liew CK, Villareal VA, Thieu W, Fadeev EA, Clemens JJ, Jung ME, Clubb RT. The structure of the Staphylococcus aureus sortase-substrate complex reveals how the universally conserved LPXTG sorting signal is recognized. J Biol Chem. 2009 Sep 04; 284(36):24465-77. view on PubMed
Fadeev EA, Sam MD, Clubb RT. NMR structure of the amino-terminal domain of the lambda integrase protein in complex with DNA: immobilization of a flexible tail facilitates beta-sheet recognition of the major groove. J Mol Biol. 2009 May 15; 388(4):682-90. view on PubMed
Wilkinson TA, Januszyk K, Phillips ML, Tekeste SS, Zhang M, Miller JT, Le Grice SF, Clubb RT, Chow SA. Identifying and characterizing a functional HIV-1 reverse transcriptase-binding site on integrase. J Biol Chem. 2009 Mar 20; 284(12):7931-9. view on PubMed
Pilpa RM, Robson SA, Villareal VA, Wong ML, Phillips M, Clubb RT. Functionally distinct NEAT (NEAr Transporter) domains within the Staphylococcus aureus IsdH/HarA protein extract heme from methemoglobin. J Biol Chem. 2009 Jan 09; 284(2):1166-76. view on PubMed
Villareal VA, Pilpa RM, Robson SA, Fadeev EA, Clubb RT. The IsdC protein from Staphylococcus aureus uses a flexible binding pocket to capture heme. J Biol Chem. 2008 Nov 14; 283(46):31591-600. view on PubMed
Yeates TO, Clubb RT. Biochemistry. How some pili pull. Science. 2007 Dec 07; 318(5856):1558-9. view on PubMed
Januszyk K, Li PW, Villareal V, Branciforte D, Wu H, Xie Y, Feigon J, Loo JA, Martin SL, Clubb RT. Identification and solution structure of a highly conserved C-terminal domain within ORF1p required for retrotransposition of long interspersed nuclear element-1. J Biol Chem. 2007 Aug 24; 282(34):24893-904. view on PubMed
Abbani MA, Papagiannis CV, Sam MD, Cascio D, Johnson RC, Clubb RT. Structure of the cooperative Xis-DNA complex reveals a micronucleoprotein filament that regulates phage lambda intasome assembly. Proc Natl Acad Sci U S A. 2007 Feb 13; 104(7):2109-14. view on PubMed
Papagiannis CV, Sam MD, Abbani MA, Yoo D, Cascio D, Clubb RT, Johnson RC. Fis targets assembly of the Xis nucleoprotein filament to promote excisive recombination by phage lambda. J Mol Biol. 2007 Mar 23; 367(2):328-43. view on PubMed
Sam MD, Abbani MA, Cascio D, Johnson RC, Clubb RT. Crystallization, dehydration and preliminary X-ray analysis of excisionase (Xis) proteins cooperatively bound to DNA. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 01; 62(Pt 8):825-8. view on PubMed
Pilpa RM, Fadeev EA, Villareal VA, Wong ML, Phillips M, Clubb RT. Solution structure of the NEAT (NEAr Transporter) domain from IsdH/HarA: the human hemoglobin receptor in Staphylococcus aureus. J Mol Biol. 2006 Jul 07; 360(2):435-47. view on PubMed
Jung ME, Clemens JJ, Suree N, Liew CK, Pilpa R, Campbell DO, Clubb RT. Synthesis of (2R,3S) 3-amino-4-mercapto-2-butanol, a threonine analogue for covalent inhibition of sortases. Bioorg Med Chem Lett. 2005 Nov 15; 15(22):5076-9. view on PubMed
Naik MT, Suree N, Ilangovan U, Liew CK, Thieu W, Campbell DO, Clemens JJ, Jung ME, Clubb RT. Staphylococcus aureus Sortase A transpeptidase. Calcium promotes sorting signal binding by altering the mobility and structure of an active site loop. J Biol Chem. 2006 Jan 20; 281(3):1817-26. view on PubMed
Pilpa RM, Clubb RT. NMR resonance assignments of the NEAT (NEAr Transporter) domain from the Staphylococcus aureus IsdH protein. J Biomol NMR. 2005 Oct; 33(2):137. view on PubMed
Iwahara J, Peterson RD, Clubb RT. Compensating increases in protein backbone flexibility occur when the Dead ringer AT-rich interaction domain (ARID) binds DNA: a nitrogen-15 relaxation study. Protein Sci. 2005 May; 14(5):1140-50. view on PubMed
Abbani M, Iwahara M, Clubb RT. The structure of the excisionase (Xis) protein from conjugative transposon Tn916 provides insights into the regulation of heterobivalent tyrosine recombinases. J Mol Biol. 2005 Mar 18; 347(1):11-25. view on PubMed
Liew CK, Smith BT, Pilpa R, Suree N, Ilangovan U, Connolly KM, Jung ME, Clubb RT. Localization and mutagenesis of the sorting signal binding site on sortase A from Staphylococcus aureus. FEBS Lett. 2004 Jul 30; 571(1-3):221-6. view on PubMed
Comfort D, Clubb RT. A comparative genome analysis identifies distinct sorting pathways in gram-positive bacteria. Infect Immun. 2004 May; 72(5):2710-22. view on PubMed
Sam MD, Cascio D, Johnson RC, Clubb RT. Crystal structure of the excisionase-DNA complex from bacteriophage lambda. J Mol Biol. 2004 Apr 23; 338(2):229-40. view on PubMed
Sam MD, Cascio D, Johnson R, Clubb RT. Crystallization and preliminary X-ray crystallographic analysis of the excisionase-DNA complex from bacteriophage lambda. Acta Crystallogr D Biol Crystallogr. 2003 Jul; 59(Pt 7):1238-40. view on PubMed
Warren D, Sam MD, Manley K, Sarkar D, Lee SY, Abbani M, Wojciak JM, Clubb RT, Landy A. Identification of the lambda integrase surface that interacts with Xis reveals a residue that is also critical for Int dimer formation. Proc Natl Acad Sci U S A. 2003 Jul 08; 100(14):8176-81. view on PubMed
Connolly KM, Smith BT, Pilpa R, Ilangovan U, Jung ME, Clubb RT. Sortase from Staphylococcus aureus does not contain a thiolate-imidazolium ion pair in its active site. J Biol Chem. 2003 Sep 05; 278(36):34061-5. view on PubMed
Milev S, Gorfe AA, Karshikoff A, Clubb RT, Bosshard HR, Jelesarov I. Energetics of sequence-specific protein-DNA association: binding of integrase Tn916 to its target DNA. Biochemistry. 2003 Apr 01; 42(12):3481-91. view on PubMed
Milev S, Gorfe AA, Karshikoff A, Clubb RT, Bosshard HR, Jelesarov I. Energetics of sequence-specific protein-DNA association: conformational stability of the DNA binding domain of integrase Tn916 and its cognate DNA duplex. Biochemistry. 2003 Apr 01; 42(12):3492-502. view on PubMed
Sam MD, Papagiannis CV, Connolly KM, Corselli L, Iwahara J, Lee J, Phillips M, Wojciak JM, Johnson RC, Clubb RT. Regulation of directionality in bacteriophage lambda site-specific recombination: structure of the Xis protein. J Mol Biol. 2002 Dec 06; 324(4):791-805. view on PubMed
Connolly KM, Iwahara M, Clubb RT. Xis protein binding to the left arm stimulates excision of conjugative transposon Tn916. J Bacteriol. 2002 Apr; 184(8):2088-99. view on PubMed
Wojciak JM, Sarkar D, Landy A, Clubb RT. Arm-site binding by lambda -integrase: solution structure and functional characterization of its amino-terminal domain. Proc Natl Acad Sci U S A. 2002 Mar 19; 99(6):3434-9. view on PubMed
Iwahara J, Iwahara M, Daughdrill GW, Ford J, Clubb RT. The structure of the Dead ringer-DNA complex reveals how AT-rich interaction domains (ARIDs) recognize DNA. EMBO J. 2002 Mar 01; 21(5):1197-209. view on PubMed
