Michael Hughes is a graduate student in David Eisenberg’s lab. Michael studied Biochemistry at Colorado State University where he did undergraduate research in the lab of Santiago Di Pietro focusing on the role of the cytoskeleton in yeast endocytosis. This undergraduate research sparked Michael’s interest in protein structure, which he now studies under the mentorship of David Eisenberg.
Michael’s research focuses on understanding the structure of mysterious regions of proteins with long repeats of amino acids called low-complexity domains. While scientists have been aware of these regions for decades, they have been historically ignored when studying proteins. Now scientists are finding that low-complexity domains are instrumental in organizing the cell and seem to be behind several amyloid diseases. Michael’s research applies diffraction techniques and computational tools to propose structures to explain how this organization occurs, where to find these structures, and potential relationships to amyloid diseases.
Kanishk Jain is a 5th-year graduate student in Dr. Steven G. Clarke’s laboratory in the Department of Chemistry and Biochemistry. Before coming to UCLA, Kanishk worked on x-ray crystallography of archaeal RNPs with Dr. Cameron Mura at the University of Virginia and received a B.S. in Chemistry with a concentration in Biochemistry.
In Dr. Clarke’s lab, Kanishk’s main focus has been to understand the biochemical mechanisms of protein arginine methyltransferases (PRMTs), enzymes that are heavily involved in epigenetic regulation of transcription by way of histone methylation. PRMT overexpression has been observed in various cancers and, as such, these enzymes are now attractive targets for drug therapies. Over the years, Kanishk’s work has uncovered determinants of PRMT product specificity and potential mechanisms of allosteric regulation of PRMT5 and PRMT7.
William Barshop is a 6th year Biological Chemistry student in the laboratory of James Wohlschlegel. William graduated from Washington University in St. Louis, where he worked on the sequencing and comparative analyses of Drosophila species under Sarah C.R. Elgin. His Ph.D. research has focused on method development and data analysis of proteomics datasets generated by mass spectrometry. These efforts have produced “MilkyWay.” MilkyWay is a label free proteomic data analysis platform for quantitative comparisons. Powered by a tapestry of tools wrapped into the Galaxy workflow engine, MilkyWay provides an interactive R/Shiny web-app interface for the definition of comparative experimental topology, upload of files, exploratory data analysis, and report export. MilkyWay provides access to quantitative interrogation of LC-MS/MS data via web interfaces, including tools for database searching, post-translational modification localization confidence estimation, intensity based quantitation, and statistical analysis for both data dependent acquisition (DDA) and data independent acquisition (DIA) datasets.
Yuxi Liu is a 6th year graduate student in the Biochemistry, Molecular, and Structural Biology PhD program working in the Todd Yeates’ lab. She is a True Bruin, graduated with the highest departmental honors from UCLA with a B.S. in Molecular, Cell, and Developmental Biology.
Symmetry operations allows protein subunits to form high-order architectures from as little as two different types of interactions. This helps explain the prevalence of grand and elegant symmetric assemblies in nature, such as viral capsids, cytoskeleton proteins, and bacterial microcompartments. A long-standing goal in nanotechnology is to create such assemblies through accurate and controllable engineering of natural proteins following the same symmetry operations. In her work in the Yeates lab, Yuxi characterized and engineered a variety of symmetric protein assemblies, including some of the largest man-made protein icosahedrons. Recently, she has re-engineered a designed tetrahedral assembly into a versatile scaffold for small proteins in cryo-EM studies.
Previous Fowler Fellows
2017 Brendan Amer (Clubb lab), Jeffrey Vinokur (Bowie lab), Anna Sahakyan (Plath lab)
2016 Henry Chan (Feigon lab), Smriti Sangwan (Eisenberg lab), Nicholas Woodall (Bowie lab)
2014 Dan McNamara (Yeates lab), Jena Quick-Cleveland (Guo lab), Nicholas Wu (Sun lab)
2013 Alex Jacobitz (Clubb lab), Alexander Patananan (S. Clarke lab), Carly Ferguson (Loo lab)
2012 Benjamin Kuryan (Carey lab), Letian Xie (C. Clarke lab), Anni Zhao (Eisenberg lab)
2011 Timothy Anderson (Clubb lab), Ian Barr (Guo lab), Soohong Kim (Weiss lab)
2010 Zeynep Durer (Reiser lab), Rachel Senturia (Guo lab), Zurita-Lopez (S. Clarke lab)
2009 Luki Goldschmidt (Eisenberg lab), Kristofer Webb (S. Clarke lab), Sheng Yin (Loo lab)
2008 Nathan Joh (Bowie lab), Neil King (Yeates lab)